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Effects of 6-aminonicotinamide and 2-deoxy-D-glucose combination on the bioenergetics of perfused Ehrlich ascites tumour cells as monitored by <sup>31</sup>P-MR spectroscopy

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Title Effects of 6-aminonicotinamide and 2-deoxy-D-glucose combination on the bioenergetics of perfused Ehrlich ascites tumour cells as monitored by 31P-MR spectroscopy
 
Creator Sharma, R K
Maitra, A
Jain, V
 
Description 307-312
The niacin antagonist,
6-aminonicotinamide (6-AN) is currently being evaluated in preclinical trials as
a potential radiosensitizer.2-Deoxy-D-glucose, a glucose antimetabolite is another promising
differential radiomodifier. In this paper we report the effects of these
antimetabolites on changes in bioenergetics of perfused immobilised Ehrlich ascites
tumour (EAT) cells to predict their usefulness in energy-linked modification of
radiation response. 31P-MRS has been used to monitor cellular energetics
as reflected in the ratio of β-phosphate of ATP to inorganic phosphate (β-ATP/Pi)
and accumulation of 6- phosphogluconate/2-deoxy-D-glucose-6-phosphate in these cells. We have earlier
shown that treatment with 2-DG results in a decrease in the energy profile of perfused
EAT cells. Presence of 6-AN alone induces a progressive decrease in the β-ATP/Pi
ratios. The impaired energy status observed after 6-AN treatment prompted us to
probe its utility in potentiating the effects of 2-DG. The combination of 6-AN and
2-DG sharply reduces the signal attributed to β-ATP to the noise levels. There was
no recovery in energy profile even upto 12 hr after perfusion with drug-free
medium. Since metabolic depletion of ATP is known to inhibit the
post-irradiation DNA repair processes and induce apoptotic cascade, the
combination may be of considerable clinical importance for optimizing the efficacy
and selectivity of tumour radiotherapy.
 
Date 2013-07-16T09:03:52Z
2013-07-16T09:03:52Z
2000-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19837
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(5) [October 2000]