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A Modified Spectrophotometric Assay of Superoxide Dismutase

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Field Value
 
Title A Modified Spectrophotometric Assay of Superoxide Dismutase
 
Creator Kakkar, Poonam
Das, Ballabh
Viswanathan, P N
 
Description 130-132
A
simple and rapid method for the assay of superoxide dismutase in biological
samples is described. Present method takes advantage of the inhibition of
NADH-dependent-nitroblue tetrazolium reduction by the dismutase. Inhibition of
the

chromogen
formation by superoxide dismutase was linear with increase in enzyme
concentrations. The chromogen extract in butanol was stable even up to 48 hr.
Superoxide dismutase activity, as assayed by the modified method, was in good
agreement with that obtained using other methods.
 
Date 2013-07-19T12:59:36Z
2013-07-19T12:59:36Z
1984-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19932
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.21(2) [April 1984]