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Identification of three novel myeloid cathelicidin cDNAs and their predicted peptides in buffalo (<i>Bubalus bubalis</i>)

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Title Identification of three novel myeloid cathelicidin cDNAs and their predicted peptides in buffalo (Bubalus bubalis)
 
Creator Panicker, Varuna P
George, Sisilamma
 
Subject Antimicrobial peptide
Buffalo
Bubalus bubalis
BuMAPs
Cathelicidin
cDNAs
 
Description 273-277
Antimicrobial peptides (AMPs) are broad spectrum
antibiotics, which mostly act without specific receptors. Identification of
AMPs is important in the current scenario of emerging multi-drug resistant
bacteria. In the present study, in an attempt to identify new AMPs, myeloid
cathelicidin cDNAs were synthesized from buffalo (Bubalus bubalis)
bone marrow and were
amplified using specific primers. Sequence analysis of cloned cDNAs revealed three
novel myeloid cathelicidins. They were named based on
the number of active amino acids in the C-terminal region of their predicted
peptide sequences as BuMAP-28 (having an additional Gly at position 22nd),
BuMAP-29 (having an additional IIe at position 27) and BuMAP-34, compared to
BMAP-27, BMAP-28 and BMAP-34 of cattle. The BuMAPs showed 93%, 95% and 87% homology respectively with that of its cattle
counterpart. Predicted number of amino acids of the cDNAs was 159, 155 and 157
residues, with cationic C-terminal sequences of 28, 29 and 34, respectively, which correspond to putative antimicrobial domains. Several amino acid substitutions were observed in all the three
cathelicidins. The conformation of the peptides was predicted to be alpha
helical, having total net positive charge and hydrophobicity, similar to that
of BMAPs in cattle. Comparative analysis of the predicted peptides suggested
potential antimicrobial activity and the sequence variations detected might
enable the peptides to act as effective broad spectrum antimicrobial agents.
 
Date 2013-09-01T13:06:03Z
2013-09-01T13:06:03Z
2013-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/20874
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(4) [August 2013]