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Airborne agricultural particulate matter induces inflammatory cytokine secretion by respiratory epithelial cells: Mechanisms of regulation by eicosanoid lipid signal mediators

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Title Airborne agricultural particulate matter induces inflammatory cytokine secretion by respiratory epithelial cells: Mechanisms of regulation by eicosanoid lipid signal mediators
 
Creator Malireddy, Smitha
Lawson, Courtney
Steinhour, Emily
Hart, Judy
Kotha, Sainath R
Patel, Rishi B
Zhao, Lingying
Wilkins, John R
Marsh, Clay B
Magalang, Ulysses J
Romberger, Debra
Wewers, Mark D
Parinandi, Narasimham L
 
Subject Organic poultry dust
Phospholipase A2
Interleukin-8
Lung epithelial cell
Prostaglandins
Leukotrienes
Occupational farm respiratory diseases
 
Description 387-401
The purpose of
this study was to elucidate the mechanism of the airborne poultry dust (particulate
matter, PM)-induced respiratory tract inflammation, a common symptom in
agricultural respiratory diseases. The study was based on the hypothesis that
poultry PM would induce the release of inflammatory cytokine interleukin-8
(IL-8) by respiratory epithelial cells under the upstream regulation by cytosolic
phospholipase A2 (cPLA2) activation and subsequent
formation of cyclooxygenase (COX)- and lipoxygenase (LOX)-catalyzed arachidonic
acid (AA) metabolites (eicosanoids). Human lung epithelial cells (A549) in
culture were treated with the poultry PM (0.1-1.0 mg) for different lengths of
time, following which PLA2 activity, release of eicosanoids and secretion
of IL-8 in cells were determined. Poultry PM (1.0 mg/ml) caused
a significant activation of PLA2 in a time-dependent manner (15-60
min), which was significantly attenuated by the
calcium-chelating agents, cPLA2-specific inhibitor (AACOCF3)
and antioxidant (vitamin C) in A549 cells. Poultry PM
also significantly induced the release of COX- and LOX-catalyzed eicosanoids
(prostaglandins, thromboxane A2 and leukotrienes B4 and C4)
and upstream activation of AA LOX in the cells. Poultry PM also significantly
induced release
of IL-8 by the cells in a dose- and time-dependent manner, which was
significantly attenuated by the calcium chelating agents, antioxidants and COX-
and LOX-specific inhibitors. The current study for the first time revealed that
the
poultry PM-induced IL-8 release from the respiratory epithelial cells was
regulated upstream by reactive oxygen species, cPLA2-, COX- and
LOX-derived eicosanoid lipid signal mediators.
 
Date 2013-10-26T09:47:09Z
2013-10-26T09:47:09Z
2013-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/22639
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(5) [October 2013]