Record Details

<span style="font-size:15.0pt;mso-bidi-font-size:16.0pt;font-weight:normal" lang="EN-US">Analysis of osmotic stress induced Ca<sup>2+</sup> spark termination <span style="font-size:15.0pt;mso-bidi-font-size:16.0pt;mso-ansi-language: EN-GB;font-weight:normal" lang="EN-GB">in mammalian skeletal muscle<span style="font-size:15.0pt; mso-bidi-font-size:16.0pt;font-weight:normal" lang="EN-US"> </span></span></span>

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Analysis of osmotic stress induced Ca2+ spark termination in mammalian skeletal muscle
 
Creator Ferrante, Christopher
Szappanos, Henrietta
Csernoch, László
Weisleder, Noah
 
Subject Calcium
Ca2+
Sparks
Calcium induced calcium release
Sarcoplasmic reticulum
Skeletal muscle
Termination
 
Description 411-418
Ca2+
sparks represent synchronous opening of the ryanodine receptor (RyR) Ca2+
release channels located at the sarcoplasmic reticulum (SR) membrane. Whereas a
quantal nature of Ca2+ sparks has been defined in cardiac muscle,
the regulation of Ca2+ sparks in skeletal muscle has not been well-studied.
Osmotic-stress applied to an intact skeletal muscle fiber can produce brief Ca2+
sparks and prolonged Ca2+ burst events. Here, we show that
termination of Ca2+ bursts occurs in a step wise and quantal manner.
Ca2+ burst events display kinetic features that are consistent with
the involvement of both stochastic attrition and coordinated closure of RyR
channels in the termination of SR Ca2+ release. Elemental unitary
transition steps could be defined with a mean DF/F0 of ~0.28, corresponding to the gating of 1-2 RyR
channels. Moreover, the amplitude of the elemental transition steps declines at
the later stage of the burst event. In tandem Ca2+ burst events
where two Ca2+ bursts occur at the same position within a fiber in
rapid succession, the trailing event is consistently of lower amplitude than
the initial event. These two complementary results suggest that SR Ca2+
release may be associated with local depletion of SR Ca2+ stores in
mammalian skeletal muscle.


 
Date 2013-10-26T10:00:29Z
2013-10-26T10:00:29Z
2013-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/22651
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(5) [October 2013]