Record Details

Expression levels of sarcolemmal membrane repair proteins following prolonged exercise training in mice

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Expression levels of sarcolemmal membrane repair proteins following prolonged exercise training in mice
 
Creator Alloush, Jenna
Roof, Steve R
Beck, Eric X
Ziolo, Mark T
Weisleder, Noah
 
Subject Caveolin-3
Dysferlin
Exercise
Membrane repair
MG53
Mitsugumin 53
Repair patch
TRIM72
Tripartite motif
Vesicle trafficking
 
Description 428-435
Membrane repair is a conserved cellular
process, where intracellular vesicles translocate to sites of plasma membrane
injury to actively reseal membrane disruptions. Such membrane disruptions
commonly occur in the course of normal physiology, particularly in skeletal
muscles due to repeated contraction producing small tears in the sarcolemmal
membrane. Here, we investigated whether prolonged exercise could produce
adaptive changes in expression levels of proteins associated with the membrane
repair process, including mitsugumin 53/tripartite motif-containing protein 72
(MG53/TRIM72), dysferlin and caveolin-3 (cav3). Mice were exercised using a
treadmill running protocol and protein levels were measured by immunoblotting.
The specificity of the antibodies used was established by immunoblot testing of
various tissue lysates from both mice and rats. We found that MG53/TRIM72
immunostaining on isolated mouse skeletal muscle fibers showed protein
localization at sites of membrane disruption created by the isolation of these
muscle fibers. However, no significant changes in the expression levels of the
tested membrane repair proteins were observed following prolonged treadmill
running for eight weeks (30 to 80 min/day). These findings suggest that any
compensation occurring in the membrane repair process in skeletal muscle
following prolonged exercise does not affect the expression levels of these
three key membrane repair proteins.
 
Date 2013-10-26T09:50:51Z
2013-10-26T09:50:51Z
2013-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/22643
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(5) [October 2013]