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Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells

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Title Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells
 
Creator Kwon, Haw-Young
Kim, Kyoung-Sook
An, Hyun-Kyu
Moon, Hyung-In
Kim, Hyun-Jun
Lee, Young-Choon
 
Subject Triptolide
U2OS cell
Apoptosis
 
Description 485-491
Triptolide,
a diterpene derived from Tripterygium wilfordii Hook f., a Chinese medicinal herb, has been reported to inhibit
cell proliferation and induce apoptosis in various human cancer cells, but its
anticancer effects on human osteosarcoma cells have not yet been elucidated. In
this study, we investigated whether triptolide induces apoptosis in human
osteosarcoma cells and the underlying molecular mechanisms. We firstly
demonstrated that triptolide inhibited cell growth and induced apoptosis in
U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2,
Bid and mitochondrial cytochrome c were
downregulated in triptolide-treated U2OS cells, whereas the levels of Fas,
FasL, Bax, cytosolic cytochrome c, cleaved
caspase-3 and cleaved PARP were upregulated. These results suggest that triptolide
induces apoptosis in U2OS cells by activating both death receptor and
mitochondrial apoptotic pathways.
 
Date 2013-12-27T11:26:05Z
2013-12-27T11:26:05Z
2013-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/25163
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(6) [December 2013]