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Lipid peroxidation and large-scale deletions of mitochondrial DNA in asthenoteratozoospermic patients

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Title Lipid peroxidation and large-scale deletions of mitochondrial DNA in asthenoteratozoospermic patients
 
Creator Abasalt, Hosseinzadeh Colagar
Gholamali, Jorsaraei Seyed
Maryam, Gholinezhad Chari
 
Subject Asthenoteratospermic
Lipid peroxidation
Malondialdehyde
Large-scale mtDNA deletions
 
Description 492-499
The correlation between malondialdehyde
(MDA) an index of lipid peroxidation (LPO) with large-scale deletion
mitochondrial DNA (mtDNA) was investigated in a case-control study with a total of 50 semen
samples from infertile men, including 25 normozoospermic donor as the control
group and 25 asthenoteratozoospermic (AT) patients as the case group. Routine
semen analysis was performed according to World Health Organization (WHO, 1999)
guidelines. MDA levels of the seminal plasma and spermatozoa were measured by
TBARS method. A long-range polymerase chain reaction (PCR) method was used for
the analysis of multiple large-scale mtDNA deletions based in two areas of
mtDNA. The results showed that mean concentration of MDA in seminal plasma (nmol/ml) and
spermatozoa (nmol/10 × 106 sperm) of AT men was higher than in
normozoospermic patients, but the differences were not statistically
significant. The products of PCR analysis showed multiple deletions of ~4.7,
4.8, 7.2, 7.3 and 7.4-kb in mtDNA of the spermatozoa in both AT and control
groups. Multiple deletions
were also observed in 64% of AT patients and 44% of the control group.
Moreover, MDA level of the spermatozoa in deleted mtDNA samples group was significantly higher than in non-deleted mtDNA group (p = 0.01). Our findings
indicated a positive correlation between increased MDA levels
and large-scale mtDNA deletions in human spermatozoa. It is suggested that LPO or other oxidative stress
factors might be causative elements in mtDNA damage, effect on sperm motility
and morphology, resulting in decline of fertility in men.


 
Date 2013-12-27T11:29:18Z
2013-12-27T11:29:18Z
2013-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/25164
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(6) [December 2013]