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Effect of dexamethasone on unfolded protein response genes (MTJ1, Grp78, Grp94, CHOP, HMOX-1) in HEp2 cell line

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Title Effect of dexamethasone on unfolded protein response genes (MTJ1, Grp78, Grp94, CHOP, HMOX-1) in HEp2 cell line
 
Creator Duzgun, Aynur
Bedir, Abdulkerim
Ozdemir, Tulay
Nar, Rukiye
Kilinc, Veli
Salis, Osman
Alacam, Hasan
Gulten, Sedat
 
Subject Grp78
CHOP
Endoplasmic reticulum stress
Dexamethasone
HEp2
Phenyl butyric acid
Lipopolysaccharide
 
Description 505-510
The endoplasmic reticulum (ER) is related to
the various signal routes that are activated in unfolded protein response
(UPR). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expressions
demonstrate UPR activity. In this study, we investigated the UPR gene
expressions in larynx epidermoid carcinoma (HEp2) to which dexamethasone (dex)
was applied. HEp2 cells were administered for 48 h with different combinations
using 0.1 μM and 1 μM dex, 1 mM phenyl butyric acid (PBA) and 100 ng/ml
lipopolysaccharide (LPS). The Grp78, Grp94, CHOP, MTJ1 and HMOX1
genes expression was determined using quantitative RT-PCR. The Grp78, MTJ1 and HMOX1 gene expression
increased with the administration of 1 µM dex. CHOP expression, on the
other hand, decreased with 0.1 µM dex. When dex was combined with LPS, nearly
all gene expressions decreased. The increase in Grp78, Grp94, HMOX1 and MTJ1
gene expression was greater in groups in which dex was administered in
combination with PBA than in groups in which dex was administered alone. Dex in low dose (0.1 μM) caused a decrease in CHOP expression in
HEp2 cells and an increase in Grp78 expression, in particular. The
changes in UPR genes expressions may lead to the extended survival of the
cells.
 
Date 2013-12-27T11:38:31Z
2013-12-27T11:38:31Z
2013-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/25168
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(6) [December 2013]