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Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga <i>Haematococcus pluvialis</i> by gibberellin A3 (GA<sub>3</sub>)

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Title Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA3)
 
Creator Gao, Zhengquan
Meng, Chunxiao
Gao, Hongzheng
Li, Yan
Zhang, Xiaowen
Xu, Dong
Zhou, Shitan
Liu, Banghui
Su, Yuanfeng
Ye, Naihao
 
Subject Haematococcus pluvialis
Astaxanthin
Gibberellin A3 (GA3)
Carotenoid genes
Real-time fluorescence quantitative PCR
 
Description 548-553
The fresh water unicellular alga Haematococcus
pluvialis is a promising natural source of astaxanthin. The present study
investigated the transcriptional expression of carotenoid genes for astaxanthin
accumulation in H. pluvialis using real-time fluorescence quantitative
PCR (qRT-PCR). With treatments of 20 and 40 mg/L of gibberllin A3
(GA3), five genes ipi-1,
ipi-2, psy, pds and bkt2 were up-regulated with
different expression profiles. GA20 (20 mg/L of GA3) treatment had a
greater effect on transcriptional expression of bkt2 than on ipi-1
ipi-2, psy and pds (>4-fold up-regulation). However,
GA40 (40 mg/L of GA3) induced more transcriptional expression of ipi-2,
psy and bkt2 than both ipi-1 and pds. The
expression of lyc, crtR-B and crtO for astaxanthin
biosynthesis was not affected by GA3 in H. piuvialis. In
the presence of GA3, astaxanthin biosynthesis genes of ipi-1, pds and bkt2 were up-regulated
at transcriptional level, psy at post-transcriptional level, whereas ipi-2
was up-regulated at both levels. The study could potentially lead to a scale
application of exogenous GA3 in astaxanthin
production with H. pluvialis just like GAs perform in
increasing crops production and it would provide new insight about the
multifunctional roles of carotenogenesis in response to GA3.


 
Date 2013-12-27T11:50:18Z
2013-12-27T11:50:18Z
2013-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/25173
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(6) [December 2013]