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<span style="mso-ansi-language:EN-US" lang="EN-US">Purification and characterization of structural and functional properties of two lectins from a marine sponge <i>Spheciospongia vesparia</i> </span>

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Title Purification and characterization of structural and functional properties of two lectins from a marine sponge Spheciospongia vesparia
 
Creator Fenton, Bertha
Espinosa, Roberto Arreguín
Contreras, Edgar Vázquez
Lozano, Barbarin Arreguín
Sánchez, Norma Sánchez
Hernández, Enrique García
Galindo, Edgar Zenteno
 
Subject Lectins
Marine sponge
Spheciospongia vesparia
Bacteriostatic activity
 
Description 562-569
The purification, structural and functional
characterization of two different lectins (named Svl-1 and Svl-2) has been
reported from the marine sponge Spheciospongia vesparia. Purification
procedure includes ammonium sulfate precipitation, combined with chromatography
including Octyl-Sepharose-(NH4)SO4 hydrophobic column and
DEAE-Toyopearl
anion-exchange column using a high performance liquid chromatography. The
similarities in function, specificity for saccharides, molecular weight, amino
acid content and the N-terminal sequence of two lectins suggest that these
proteins are isolectins. Amino acid composition and fluorescence analyses
reveal that they contain an intrachain disulfide bridge, which might contribute
to their high thermal stability. Furthermore, the purified lectins exhibit
antibacterial activity against the gram-negative bacteria Pseudomonas
aeruginosa and E. coli, indicating that they may be involved in a
recognition strategy and may play a role in the defense response function of
the sponge. This is the first report on the isolation of lectins from the S.
vesparia. The purified lectins represent a potential possible candidate for
future application in the recognition or treatment of cancer cells.
 
Date 2013-12-27T12:04:33Z
2013-12-27T12:04:33Z
2013-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/25175
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(6) [December 2013]