Record Details

Study of reaction parameters and kinetics of esterification of lauric acid with butanol by immobilized <i>Candida antarctica</i> lipase

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Study of reaction parameters and kinetics of esterification of lauric acid with butanol by immobilized Candida antarctica lipase
 
Creator Shankar, Sini
Agarwal, Madhu
Chaurasia, S P
 
Subject Esterification
Butyl laurate
Immobilized
Candida antarctica lipase
 
Description 570-576
Esterification
of lauric acid with n-butanol, catalyzed by immobilized Candida
antarctica lipase (CAL)
in aqueous-organic biphasic solvent system was studied. Effects of various
reaction parameters on esterification were investigated,
such as type and amount of solvent, amount of buffer, pH, temperature, speed of
agitation, amount of enzyme, butanol and lauric acid. The most suitable
reaction conditions for esterification were observed at 50ºC and pH 7.0 using
5000 μmoles of lauric acid, 7000 μmoles of butanol, 0.25 ml phosphate buffer, 1 ml of isooctane as the
solvent and 50 mg of immobilized enzyme in the reaction medium at agitation
speed of 150 rpm. Maximum esterification of 96.36% was acheived in 600 min of
reaction time at n-butanol to lauric acid molar ratio of 1: 0.7. Kinetic
study for the esterification of lauric acid with
n-butanol using immobilized CAL
was carried out and the kinetic constants were estimated by using non-linear
regression method. The estimated value of Michaelis kinetic constants for
butanol (KmBt) and acid (KmAc) were 451.56
(M) and
4.7 × 10-7(M), respectively and the value of dissociation constant (KBt)
of the butanol-lipase complex was 9.41 × 107(M). The estimated
constants agreed fairly well with literature data.
 
Date 2013-12-27T11:36:26Z
2013-12-27T11:36:26Z
2013-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/25167
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(6) [December 2013]