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Study of photodynamic, sonodynamic and antioxidative influence on HeLa cell line

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Title Study of photodynamic, sonodynamic and antioxidative influence on HeLa cell line
 
Creator Tomankova, Katerina
Kolarova, Hana
Vachutka, Jaromir
Zapletalova, Jana
Hanakova, Adela
Kaplova, Eva
 
Subject Photodynamic treatment
Sonodynamic treatment
Antioxidant
Reactive oxygen species
Atomic force microscopy
 
Description 19-28
Photodynamic treatment (PDT) in combination
with sonodynamic treatment (SDT) can be used as suitable methods to treat
malignant and benign diseases or combat resistant bacteria. Both methods affect
the production of reactive oxygen species (ROS). On the other hand,
antioxidants are useful for cell protection against ROS. This work was aimed to
study the effect of PDT and SDT treatments on the HeLa cell line using
antioxidant Pronalen Sensitive Skin® as a protection from free radicals in the
cells. We evaluated the effect of sensitizer ClAlPcS2 using battery
of in vitro methods, including MTT assay, kinetic production of ROS,
mitochondrial membrane potential change, type of cell death and microscopic
analysis. Ultrasound treatment was observed to increase the production of ROS,
only in combination with PDT, particularly at higher concentrations of ClAlPcS2.
The added antioxidant acts as protection against free radicals and has
potential as a dietary supplement against aging or free radicals. The results
of study suggested that ClAlPcS2 could be used as a potential
photosensitizer for treatment of a specific type of cancers.
 
Date 2014-03-03T17:53:28Z
2014-03-03T17:53:28Z
2014-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/27294
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(1) [February 2014]