Record Details

<i>Azadirachta indica </i>mitigates<i> </i>DMBA-induced hepatotoxicity: A biochemical and radiometric study

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Azadirachta indica mitigates DMBA-induced hepatotoxicity: A biochemical and radiometric study
 
Creator Koul, Ashwani
Mohan, Vandana
Bharati, Sanjay
 
Subject Azadirachta indica
Hepatotoxicity
Carcinogen metabolism
Oxidative stress
7,12-Dimethylbenz[a] anthracene
Liver function
 
Description 37-45
The hepatoprotective potential of aqueous Azadirachta
indica leaf extract (AAILE) was assessed against DMBA-induced
hepatotoxicity. DMBA  (7,12-dimethylbenz[a]
anthracene) treatment (40 mg/kg body weight, ip) to male Balb/c mice resulted
in the derailment of liver function as revealed by extremely slow clearance of 99mTc-mebrofenin
from liver, elevated levels of alkaline phosphatase (ALP) and alanine
transaminase (ALT), compared to control group. In addition, elevated micronuclei
score and high apoptotic index indicated hepatogenotoxicity in DMBA-treated
mice. DMBA treatment also upregulated cytochrome P450 (CYP), cytochrome b5
(Cyt b5) and decreased glutathione-S-transferase activity in hepatic
tissue, compared to control group. Enhanced lipid peroxidation (LPO) levels
along with decreased reduced glutathione (GSH) level were also observed in DMBA
group, compared to control group. AAILE co-treatment (200 mg/kg body weight,
po, thrice a week) for 8 weeks followed by DMBA injection showed significant
improvement in hepatic status, as revealed by normalization of 99mTc-mebrofenin
clearance rate, decreased ALP and ALT levels, reduced genotoxicity in terms of
micronuclei score and apoptotic index. Levels of LPO were significantly decreased
along with increased hepatic GST and GSH levels in AAILE + DMBA group, compared
to DMBA group. However, no significant change was observed in hepatic CYP and
Cyt b5 levels, compared to DMBA group. The results indicated that
AAILE effectively ameliorated
DMBA-induced hepatotoxicity.
 
Date 2014-03-03T17:43:12Z
2014-03-03T17:43:12Z
2014-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/27286
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(1) [February 2014]