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<i style="mso-bidi-font-style:normal">In vitro</i> cytotoxic activity of leaves extracts of <i style="mso-bidi-font-style:normal">Holarrhena antidysenterica </i>against some human cancer cell lines

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Title In vitro cytotoxic activity of leaves extracts of Holarrhena antidysenterica against some human cancer cell lines
 
Creator Sharma, Vikas
Hussain, Shabir
Bakshi, Manish
Bhat, Neha
Saxena, Ajit Kumar
 
Subject Holarrhena antidysenterica
Cytotoxic activity
Human cancer cells
SRB assay
 
Description 46-51
In
vitro cytotoxic potential of extracts (95% and 50%
ethanolic extract and hot water extract at concentration of
100 µg/ml) from leaves of Holarrhena
antidysenterica was evaluated against fourteen human cancer cell lines —
A-549, COLO-205, DU-145, HeLa, HEP-2, IMR-32, KB, MCF-7, NCI-H23, OVCAR-5,
SiHa, SK-N-MC, SW-620 and ZR-75-1 from nine different tissues (breast, colon,
cervix, CNS, lung, liver, oral, ovary and prostate) using SRB assay. The 95%
ethanolic extract displayed maximum anti-proliferative effect
in the range of 73-92% against eight human cancer cell lines, while 50%
ethanolic extract showed cytotoxic activity in the range of 70-94% against
seven human cancer cell lines. However, the hot water extract did not show any
activity. Among the fractions of 95% and 50% ethanolic extract, significant
cytotoxic activity was found in the chloroform soluble fraction of 95%
ethanolic extract at 100 µg/ml; it inhibited the growth in the range of 71-99%
of seven human cancer cell lines from five different tissues viz., OVCAR-5 (ovary), HT-29 (colon),
SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary)
and A-549 (lung). The cytotoxic activity of chloroform soluble fraction was
found to be higher than 5-flurouracil, adriamycin, mitomycin-c and paclitaxel
(anticancer drugs used as positive controls). Further in vivo studies and identification of active components from the
chloroform fraction and their exact mechanism of action could be useful in
designing new anticancer therapeutic agents.
 
Date 2014-03-03T17:44:21Z
2014-03-03T17:44:21Z
2014-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/27287
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(1) [February 2014]