Record Details

Incorporation of Na<sup>+</sup>/H<sup>+</sup> antiporter gene from <i style="mso-bidi-font-style:normal">Aeluropus littoralis </i>confers salt tolerance in soybean (<i style="mso-bidi-font-style:normal">Glycine max</i> L.)

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Incorporation of Na+/H+ antiporter gene from Aeluropus littoralis confers salt tolerance in soybean (Glycine max L.)
 
Creator Liu, Jianfeng
Zhang, Shuling
Dong, Lijun
Chu, Jianzhou
 
Subject Soybean
Glycine max L.
Ovary-drip transformation
Na+/H+ antiporter gene
Transgenic plants
Salt-tolerance
Aeluropus littoralis
 
Description 58-65
To develop a salt-tolerant soybean (Glycine max L.) cultivar,
a minimal linear Na+/H+ antiporter gene cassette (35S
CaMV promoter, open-reading-frame of AlNHX1 from Aeluropus littoralis and NOS terminator)
was successfully expressed in soybean cultivar TF-29. Southern and Northern
blot analysis showed that AlNHX1 was successfully incorporated into the
genome and expressed in the transgenic plants. The AlNHX1 transgenic plant lines exhibited improved growth in severe
saline condition (150 mM
NaCl). The transgenic lines accumulated a lower level of Na+ and a
higher level of K+ in the leaves than wild-type plants under saline
condition (150 mM
NaCl). Observations on the chlorophyll content, photosynthetic rates,
malondialdehyde and relative electrical conductivity indicated that transgenic
plants exhibited tolerance to salt stress, growing normally at
salt concentrations up to 150 mM. These
results demonstrated that AlNHX1 was successfully
transferred into soybean and the salt-tolerance was improved by the
overexpression of AlNHX1.
 
Date 2014-03-03T17:45:18Z
2014-03-03T17:45:18Z
2014-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/27288
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(1) [February 2014]