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<span style="font-size:15.0pt;mso-bidi-font-size:14.0pt;color:black" lang="EN-US">Analysis of water soluble polysaccharides as a potential chemotaxonomic marker for landraces in <i style="mso-bidi-font-style:normal">Bixa orellana</i> </span>

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Title Analysis of water soluble polysaccharides as a potential chemotaxonomic marker for landraces in Bixa orellana
 
Creator Parimalan, Rangan
Mahendranath, Gondi
Giridhar, Parvatam
 
Subject Annatto
Bixa orellana L.
Bixin
Chemotaxonomic markers
Polysaccharide
Landraces
 
Description 81-86
Annatto tree (Bixa orellana L.) is
native to Brazil and is now under cultivation in many parts of world for its
reddish orange ‘annatto’ dye. There are three types of landraces in annatto and
they are distinguished based on fruit shape i.e., ovate, conical and
hemispherical, whose pigment yield differs. Since annatto pigment yield varies
with landrace, it is necessary to characterize markers towards the
identification of landraces. In this study, we characterized water soluble
polysaccharides (WSP) of twigs from three landraces using size-exclusion
chromatography (SEC), fourier-transform Infrared spectroscopy (FT-IR), nuclear
magnetic resonance spectroscopy (NMR) and gas liquid chromatography (GLC) for
their potential
use as chemotaxonomic markers to distinguish the landraces. GLC analysis on WSP
showed hemispherical type contained 38% rhamnose, while conical and ovate types
contained 17% and 34% glucose, respectively. Thus, glucose and rhamnose content
of WSP could be used to distinguish the three landraces. Further, differences
in calculated molecular weight as revealed by SEC (281.8, 151.3 and 79.4 kDa
for conical, hemispherical and ovate types, respectively) could also be used to
distinguish the three landraces.
 
Date 2014-03-03T17:50:32Z
2014-03-03T17:50:32Z
2014-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/27291
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(1) [February 2014]