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<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:SimSun;mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Molecular cloning and characterization of Pseudorabies virus <i style="mso-bidi-font-style: normal">EP0</i> gene</span>

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Title Molecular cloning and characterization of Pseudorabies virus EP0 gene
 
Creator Li, Mei-li
Cui, Wei
Zhao, Zhi-yao
Mo, Chun-cong
Wang, Jin-lin
Chen, Ya-lan
Cai, Ming-sheng
 
Subject Pseudorabies virus
EP0
Cloning
Bioinformatics
Molecular characterization
 
Description 100-114
The
pseudorabies virus (PRV) early protein
EP0 is a homologue of the herpes simplex virus 1 (HSV-1) immediate-early protein ICP0, which is a multifunctional protein and important for HSV-1
infection. However, the exact function of EP0 is not clear. In this study, using
polymerase chain reaction, a 1,104 base-pair sequence of the EP0 gene was amplified from the PRV
Becker strain genome and identification of the EP0 gene was confirmed by further cloning and sequencing.
Bioinformatics analysis indicated that the PRV EP0 gene encoded a putative polypeptide with 367 amino acids. The
encoded protein, designated as EP0 contained a conserved RING-finger
superfamily domain and was found
to be closely related with the herpes virus RING-finger superfamily and was
highly conserved among the counterparts encoded by RING-finger genes. Multiple nucleic acid sequence and amino-acid
sequence alignments suggested that PRV EP0 showed a relatively higher
similarity with EP0-like proteins of genus Varicellovirus than with those of other
genera of Alphaherpesvirinae. In addition, phylogenetic analysis
showed that PRV EP0 had a close evolutionary relationship with members of genus Varicellovirus, especially bovine
herpesvirus 1 (BoHV-1) and BoHV-5. Antigen prediction indicated that several
potential B-cell epitopes were located in EP0. Also, subcellular localization
analysis demonstrated that EP0 was predominantly localized in the nucleus,
suggesting that it might function as a nuclear-targeted protein.


 
Date 2014-04-30T06:33:20Z
2014-04-30T06:33:20Z
2014-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/28687
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(2) [April 2014]