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<span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Anti-proliferative and apoptotic properties of a peptide from the seeds of <i>Polyalthia longifolia</i> against human cancer cell lines</span>

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Title Anti-proliferative and apoptotic properties of a peptide from the seeds of Polyalthia longifolia against human cancer cell lines
 
Creator Rupachandra, S
Sarada, D V L
 
Subject Cytotoxicity
Flow cytometer
LC-ESI- MS/MS analysis
MTT assay
 
Description 127-134
The peptides produced enzymatically from
various plants have shown various biological activities including cytotoxicity.
Different types of cytotoxic peptides have been reported from the seeds and
leaves of Violaceae, Rubiaceae and Annonaceae families. In this study, we
report purification and characterization of peptide(s) showing cytotoxic
activity against A549 and HeLa cancer cell lines from the seeds of Polyalthia
longifolia (Annonaceae). Seed proteins of P. longifolia were
extracted and hydrolyzed using trypsin. The enzyme hydrolysate was applied on
to a Sephadex G10 column and eluted using
Tris-HCl buffer (pH 7.5). Two fractions F1 and F2 were obtained, of which F2
showed significant cytotoxic activity against lung (A549) cancer cells at 10
µg/mL and cervical (HeLa) cancer cell lines at 30 µg/mL, as revealed by the MTT
assay. DNA fragmentation was observed in the tested cancer cell lines treated
with F2 peptide at a concentration of 10 µg/mL and 30 µg/mL, respectively.
Further, increased number of apoptotic cells was observed in sub-G0 phase of
cell cycle of A549 and HeLa cell lines, when treated with 10 µg/mL and 30 µg/mL
of F2, as revealed by the flow cytometric analyses. FTIR spectrum of F2 peptide
detected the presence of stretching vibrations of carboxylic acid OH residue
with peak at 3420 cm-1 and carbonyl (C=O) groups at 1636 cm-1,
respectively. RP-HPLC analysis of F2 peptide showed a single peak at a
retention time of 12.8 min detected at 280 nm, depicting the purity of F2 to be
more than 90%. LC-ESI-MS/MS analysis showed the average theoretical mass of F2
to be 679.8 using m/z ratios. In conclusion, the findings suggest that F2
peptide is an effective inducer of apoptosis of cancer cells, thus offers an
important strategy in the development of
cancer therapeutics.
 
Date 2014-04-30T06:43:02Z
2014-04-30T06:43:02Z
2014-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/28690
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(2) [April 2014]