Record Details

<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:JA;mso-bidi-language:HI" lang="EN-GB">Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a Bangladeshi cultivar of potato (<i>Solanum tuberosum</i>)</span>

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Title Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a Bangladeshi cultivar of potato (Solanum tuberosum)
 
Creator Hasan, Imtiaj
Ozeki, Yasuhiro
Kabir, Syed Rashel
 
Subject N-Acetyl D-Glucosamine
Antibiofilm
Antimicrobial
Chitin
Lectin
Solanum tuberosum
 
Description 142-148
A new chitin-binding lectin was purified from a Bangladeshi cultivar ‘Deshi’ of potato (Solanum tuberosum L.) through
anion-exchange and affinity chromatographies using a chitin column. Sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed the molecular
mass of the lectin as 20,000 Daltons.
This molecular mass was almost half of the molecular masses of chitin-binding
lectins derived from other potatoes. The lectin showed both bactericidal and
growth-inhibiting activities against Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli, Salmonella enteritidis and Shigella boydii) pathogenic bacteria.
It also showed antifungal activity against Rhizopus spp., Penicillium spp. and Aspergillus niger. Biofilm
produced by the bacterium Pseudomonas aeruginosa was dose-dependently reduced by 5-20% in 24 h after administration of the
lectin, which was attributed to the glycan-binding property of the lectin
having affinity to GlcNAc polymers. It was the first observation that any
potato lectin prevented biofilm formation by
P. aeruginosa and, therefore, could have possible
applications in clinical microbiology and biomedical science.
 
Date 2014-04-30T06:59:13Z
2014-04-30T06:59:13Z
2014-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/28692
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(2) [April 2014]