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<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Cloning and characterization of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) gene from <i style="mso-bidi-font-style:normal">Paris</i><i style="mso-bidi-font-style:normal"> fargesii </i>Franch</span>

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Title Cloning and characterization of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) gene from Paris fargesii Franch
 
Creator Liang, Yili
Jiang, XueMei
Hu, Qi
Li, Xiaoqi
HuaqunYin
Li, Diqiang
Zhang, Yuguang
Liu, Xueduan
 
Subject Paris fargesii Franch
3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR)
Cloning
RACE
 
Description 201-206
3-Hydroxy-3-methylglutaryl-coenzyme A reductase
(HMGR) plays an important role in catalyzing the first committed step of isoprenoids biosynthesis in mevalonic acid (MVA) pathway. Here, we cloned a full-length
transcript of Paris fargesii Franch. The full-length cDNA of P. fargesii HMGR (Pf-HMGR, GenBank accession no.
JX508638) was 1,973 bp and contained a 1,728 bp ORF encoding 576 amino acids.
Sequence analysis revealed that the deduced Pf-HMGR had high similarity with
HMGRs from other plants, including
Ricinus communis (77%), Litchi
chinensis (76%), Michelia chapensis
(75%) and Panax quinquefolius (72%).
It had a calculated molecular mass of about 62.13 kDa and an isoelectric point
(pI) of 8.47. It contained two transmembrane domains, two putative HMGR binding
sites and two NADP(H)-binding sites. The predicted 3-D structure revealed that
Pf-HMGR had a similar spatial structure with other plant HMGRs. Three catalytic
regions, including L-domain, N-domain and S-domain were detected by structural
modeling of HMGR. Tissue expression analysis revealed that Pf-HMGR was strongly
expressed in roots and stems than in leaves. Taken together, our data laid a
foundation for further investigation of HMGR's functions and regulatory
mechanisms in plants.
 
Date 2014-07-14T05:52:05Z
2014-07-14T05:52:05Z
2014-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/29084
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(3) [June 2014]