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<i>nspA</i> Gene as a specific genetic marker for detection of <i>Neisseria meningitidis</i> causing bacterial meningitis

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Title nspA Gene as a specific genetic marker for detection of Neisseria meningitidis causing bacterial meningitis
 
Creator Bhatt, Neha
Khan, Nazneen
Dash, Sandip K
Khare, Shashi
Kumar, Ashok
 
Subject Genetic marker
Meningitis
Neisseria meningitidis
nspA gene
 
Description 211-214
Bacterial
meningitis caused by Neisseria meningitidis which causes human brain
meninges damage, is generally diagnosed from patient cerebrospinal fluid
through microscopy, immunological assays, biochemical test, PCR, microarray and
biosensors. However, these methods are expensive, time-consuming or
non-confirmatory due to certain limitations. A quick PCR based method was
developed for detection of bacterial meningitis caused by N. meningitidis
using specific primers based on amplification of virulence nspA (Neisseria
surface protein A) gene partial sequence (202 bp). The nspA gene
amplicon could be used as a genetic marker for minimum detection of 10 ng
genomic DNA (G-DNA) of N. meningitidis with high sensitivity only in 80
min, which is least time reported for the confirmation of the disease. However,
the lower detection limit was found as low as 1.0 ng G-DNA, but with less sensitivity.
The cross-reactivity of the genetic marker was also studied with other possible
pathogens. A comparison with the presently available detection methods and our
method was also done using patient samples.
 
Date 2014-07-14T05:54:04Z
2014-07-14T05:54:04Z
2014-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/29086
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(3) [June 2014]