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Construction of cDNA expression library of watermelon for isolation of <i>ClWRKY1 </i>transcription factors gene involved in resistance to Fusarium wilt

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Title Construction of cDNA expression library of watermelon for isolation of ClWRKY1 transcription factors gene involved in resistance to Fusarium wilt
 
Creator Yang, Bing-Yan
Huo, Xiu-Ai
Li, Peng-Fei
Wang, Cui-Xia
Duan, Hui-Jun
 
Subject cDNA library
Citrullus lanatus Thunb
Watermelon (PI296341)
Fusarium wilt
ClWRKY1 gene
 
Description 302-307
Full-length cDNAs are very important for genome annotation and
functional analysis of genes. The number of full-length cDNAs from watermelon
remains limited. Here we report first the construction of a full-length
enriched cDNA library from Fusarium wilt stressed watermelon (Citrullus
lanatus Thunb.) cultivar PI296341 root tissues using the SMART method. The
titer of primary cDNA library and amplified library was 2.21 × 106
and 2.0 × 1010 pfu/ml, respectively and the
rate of recombinant was above 85%. The size of insert fragment ranged from 0.3
to 2.0 kb. In this study, we first cloned a gene named ClWRKY1, which
was 1981 bp long and encoded a protein consisting of 394 amino acids. It
contained two characteristic WRKY domains and two zinc finger motifs.
Quantitative real-time PCR showed that ClWRKY1 expression levels reached
maximum level at 12 h after
inoculation with Fusarium oxysporum f. sp. niveum. The
full-length cDNA library of watermelon root tissues is not only essential for
the cloning of genes which are known, but also an initial key for the screening
and cloning of new genes that might be involved in resistance to Fusarium wilt.
 
Date 2014-09-03T04:20:06Z
2014-09-03T04:20:06Z
2014-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/29324
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(4) [August 2014]