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Cell cycle inhibitory activity of <i style="mso-bidi-font-style:normal">Piper longum</i> against A549 cell line and its protective effect against metal-induced toxicity in rats

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Title Cell cycle inhibitory activity of Piper longum against A549 cell line and its protective effect against metal-induced toxicity in rats
 
Creator Sharma, Amit Kumar
Kumar, Shashank
Chashoo, Gousia
Saxena, Ajit K
Pandey, Abhay K
 
Subject Cytotoxicity
Cell cycle
Piper longum
Lipid peroxidation
Hepatoprotective
Hepatotoxicity
Serum enzymes
 
Description 358-364
Anticancer potential of Piper longum fruit against human cancer cell lines (DU-145 prostate, A549
lung, THP-1 leukemia, IGR-OVI-1 ovary and MCF-7 breast)
as well as its in vitro and in vivo biochemical efficacy in AlCl3-induced
hepatotoxicity were evaluated in the rats. Dried samples were extracted with
several solvents using soxhlet apparatus. Flavonoid content in chloroform,
benzene, ethyl alcohol and aqueous extracts of fruit was 19, 14, 12 and 11 μg quercetin equivalent/mg
of sample, respectively. Hexane extracts exhibited 90-92%
cytotoxicity against most of the test cell lines (A549, THP-1, IGR-OVI-1
and MCF-7),
while benzene extract displayed 84-87% cytotoxicity against MCF-7, IGR-OV-1 and
THP-1 cell lines. Among extracts, hexane, benzene and acetone extracts
demonstrated considerable cytotoxicity
(91-95%) against A549 (lung cancer) cell line in Sulforhodamine B dye (SRB)
assay. Cell cycle analysis revealed that hexane, benzene and acetone extracts
produced 41, 63 and 43% sub-G1 DNA fraction, demonstrating cell cycle
inhibitory potential of these extracts against A549 cell line. Chloroform,
ethyl alcohol and aqueous extracts displayed 71, 64 and 65% membrane protective
activity, respectively in lipid
peroxidation inhibition assay. P. longum fruit
extracts also ameliorated AlCl3-induced hepatotoxicity, as indicated
by alterations observed in serum enzymes ALP, SGOT and SGPT activity, as well as creatinine
and bilirubin contents. In conclusion, study established the cytotoxic and
hepatoprotective activity in
P. longum extracts.
 
Date 2014-11-12T10:59:03Z
2014-11-12T10:59:03Z
2014-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/29884
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(5) [October 2014]