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Exogenous application of putrescine at pre-anthesis enhances the thermotolerance of wheat (<i>Triticum aestivum </i>L.)

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Title Exogenous application of putrescine at pre-anthesis enhances the thermotolerance of wheat (Triticum aestivum L.)
 
Creator Kumar, Ranjeet R
Sharma, Sushil K
Rai, Gyanendra K
Singh, Khushboo
Choudhury, Madhumanthi
Dhawan, Gaurav
Singh, Gyaneshwar P
Goswami, Suneha
Pathak, Himanshu
Rai, Raj D
 
Subject Heat stress
Antioxidants
Putrescine
Wheat
Antioxidant enzymes
Quantitative real-time PCR
Heat shock proteins
Triticum aestivum L.
 
Description 396-406
Antioxidant enzymes, besides being involved
in various developmental processes, are known to be important for environmental
stress tolerance in plants. In this study, the effect of treatment of 2.5 mM
putrescine (Put), heat stress
(HS -42°C for 2 h) and their combination on the expression and activity of
antioxidant enzymes was studied at pre-anthesis in the leaves of two wheat (Triticum
aestivum L.) cultivars — HDR77 (thermotolerant) and HD2329
(thermosusceptible). We observed that 2.5 mM Put before HS significantly
enhanced the transcript levels of superoxide dismutase (SOD), catalase (CAT),
cytoplasmic and peroxisomal ascorbate peroxidase (cAPX, pAPX) in
both the cultivars. However, the activities of antioxidant enzymes (SOD, CAT,
APX and GR), as well as accumulation of antioxidants (ascorbic acid and total
thiol content) were higher in HDR77 than in HD2329 in response to the treatment
2.5 mM Put + HS. No significant change was observed in the proline
accumulation in response to HS and combined treatment of 2.5 mM Put +
HS.
A decrease in the H2O2 accumulation, lipid peroxidation
and increase in cell membrane stability (CMS) were observed in response to 2.5
mM Put + HS treatment, as compared to HS treatment alone in both the
cultivars; HDR77 was, however, more responsive to 2.5 mM Put + HS
treatment. Put (2.5 mM) treatment at pre-anthesis thus modulated the defense
mechanism responsible for the thermotolerance capacity of wheat under the heat
stress. Elicitors like Put, therefore, need to be further studied for
temporarily manipulating the thermotolerance capacity of wheat grown under the
field conditions in view of the impending global climate change.
 
Date 2014-11-12T11:03:44Z
2014-11-12T11:03:44Z
2014-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/29888
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(5) [October 2014]