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<span style="mso-bidi-language:HI">Characterization of carotenoid hydroxylase gene promoter in <i>Haematococcus pluvialis</i> </span>

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Title Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis
 
Creator Meng, C X
Wei, W
Su, Z-L
Qin, S
 
Subject Haematococcus pluvialis
Astaxanthin
Carotenoid hydroxylase
cis-Acting element
Promoter
 
Description 284-288
Astaxanthin, a high-value ketocarotenoid is mainly
used in fish aquaculture. It also has potential in human health due to its
higher antioxidant capacity than β-carotene and vitamin E, The unicellular
green alga Haematococcus pluvialis is known

to accumulate astaxanthin in response to
environmental stresses, such as high light intensity and salt stress.
Carotenoid hydroxylase plays a key role in astaxanthin biosynthesis in H.
pluvialis. In this paper, we report the characterization of a

promoter-like region (-378 to -22 bp) of
carotenoid hydroxylase gene by cloning, sequence analysis and functional verification
of its 919 bp 5'-flanking region in H. pluvialis. The 5’-flanking
region was characterized using micro-particle

bombardment method and transient expression of LacZ
reporter gene. Results of sequence analysis showed that the 5’-flanking
region might have putative cis-acting elements, such as ABA (abscisic
acid)-responsive element (ABRE), Crepeat/

dehydration responsive element (C-repeat/DRE),
ethylene-responsive element (ERE), heat-shock element (HSE), wound-responsive
element (WUN-motif), gibberellin-responsive element (P-box), MYB-binding site
(MBS) etc., except for typical TATA and CCAAT boxes. Results of 5' deletions
construct and β-galactosidase assays revealed that a highest promoter-like
region might exist from -378 to -22 bp and some negative regulatory elements
might lie in the region from -919 to -378 bp. Results of site-directed
mutagenesis of a putative C-repeat/DRE and an ABRE-like motif in the
promoterlike region (-378 to -22 bp) indicated that the putative C-repeat/DRE
and ABRE-like motif might be important for expression of carotenoid hydroxylase
gene.
 
Date 2015-01-16T10:41:51Z
2015-01-16T10:41:51Z
2006-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30373
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.43(5) [October 2006]