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Variant of mitochondrial ribosomal protein s28 (mrps28) gene is differentially expressed in response to radiation in a cervical carcinoma derived cell line

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Title Variant of mitochondrial ribosomal protein s28 (mrps28) gene is differentially expressed in response to radiation in a cervical carcinoma derived cell line
 
Creator Gopal, G
Rajkumar, T
 
Subject Cervix cancer
SiHa cells
Radiation response
Differential display
Mitochondrial ribosomal proteins
MRPS28
kDec03 MRPS28
Splice variants MRPS
Reverse transcriptase polymerase chain reaction
Differential expression
 
Description 81-86
Gene expression before and after radiation
treatment (10 Gy) in a cervical carcinoma derived cell line SiHa was studied.
Differential display performed on non-irradiated and irradiated cells (10 Gy)
using a combination of 3 anchor primers and 16 arbitrary primers yielded 20
differentially expressed fragments. Differential expression of one of the fragments
differentially expressed In the irradiated sample was also confirmed in
Northern blots. Cloning and sequencing of the fragment revealed it to be
similar to a splice variant of the MRPS28 gene. Reverse transcriptase
polymerase chain

reaction (RT-PCR) was performed using primers
designed to amplify the splice variant and confirmed the expression. In conclusion,
the study shows that the splice variant kDec03 of the MRPS28 gene is
differentially expressed in response to

radiation in SiHa cells.


 
Date 2015-01-16T11:33:31Z
2015-01-16T11:33:31Z
2005-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30379
 
Language en_US
 
Relation C12N15/12
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.42(2) [April 2005]