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Studies on surfactant-biopolymer interaction. II. Interaction of cetyl trimethyl ammonium-, cetyl ethanolyl dimethyl ammonium-, cetyl diethanolyl methyl ammonium- and cetyl triphenyl phosphonium bromides and cetyl pyridinium chloride with calf thymus DNA

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Title Studies on surfactant-biopolymer interaction. II. Interaction of cetyl trimethyl ammonium-, cetyl ethanolyl dimethyl ammonium-, cetyl diethanolyl methyl ammonium- and cetyl triphenyl phosphonium bromides and cetyl pyridinium chloride with calf thymus DNA
 
Creator Chatterjee, Arindam
Moulik, Satya P
 
Subject Surfactants interaction
Calf thymus DNA
Spectrophotometry
Viscometry
Tensiometry
Dynamic light scattering
Circular dichroism
Fluorescence microscopy
Isothermal titration calorimetry
 
Description 205-215
The interaction of the surfactants cetyl trimethyl
arnrnonium-, cetyl ethanolyl dimethyl ammonium-, cetyl diethanolyl methyl
arnmonium-, and cetyl triphenyl phosphonium bromides and cetyl pyridinium
chloride with calf thymus DNA was

studied at 303 K in phosphate buffer (pH
7.0) at 10 mM NaCI using
spectrophotometric, viscometric, tensiometric, dynamic light scattering,
circular dichroism, fluorescence microscopic and microcalorimetric techniques.
All the surfactants interacted fairly with DNA, making the biopolymer
condensed, even to the aggregated globular configuration at higher [surfactant]/[DNA]
mole ratio (R), with direct evidence from fluorescence microscopy. Melting
temperature and light

scattering intensity of the DNA increased, whereas
viscosity decreased in the presence of the surfactants. Tensiometry evidenced
effective interaction at [surfactant] as low as 7.6 ยตM. Isothermal
titration calorimetric measurements supported low enthalpy of binding, induced
aggregation of the surfactants, increased critical micellar concentration and
association of aggregates with the biopolymer at higher R, evidencing
distinctions in thermal behaviour.
 
Date 2015-01-16T11:36:49Z
2015-01-16T11:36:49Z
2005-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30382
 
Language en_US
 
Relation G01K3/00, G01N33/52
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.42(4) [August 2005]