Record Details

<span style="mso-bidi-language:HI">Molecular and biochemical markers associated with leaffolder (<i>Cnaphalocrocis medinalis </i>G.) resistance in rice (<i>Oryza sativa </i>L.) </span>

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Molecular and biochemical markers associated with leaffolder (Cnaphalocrocis medinalis G.) resistance in rice (Oryza sativa L.)
 
Creator Sinha, Seema
Balasaraswathi, R
Selvaraju, K
Shanmugasundaram, P
 
Subject Rice
Oryza sativa L
Simple sequence repeat
Isozyme
Leaffolder
Cnaphalocrocis medinalis G.
Peroxidase
Phenylalanine ammonia lyase
SDS-PAGE
Bulk segregant analysis
Leaf protein profile
Soluble protein
 
Description 228-232
Association of
molecular markers namely isozymes and simple sequence repeats (SSRs) and
various biochemical markers to leaffolder (Cnaphalocrocis medinalis G.,
a predominant insect pest of rice) resistance were studied in rice (Oryza
sativa L.). Recombinant inbred lines (RILs) of F8 generation
obtained by crossing IR36 (susceptible parent) and TNAULFR831311 (moderately
resistant parent) were used in this study. Soluble protein content, protein
profile, and peroxidase and phenylalanine ammonia lyase (PAL) activities were
the various biochemical markers studied. Decrease in soluble leaf protein
content was observed in all lines, due to insect infestation. Protein profiling
revealed an enhanced expression of a high molecular mass (>97 kDa) protein
in all the infested lines. Besides, there was an increased induction of a 38
kDa protein in infested resistant parent and resistant RILs. A significant
increase in peroxidase and PAL activities was observed after infestation. In
peroxidase isozyme analysis, carried out after infestation, "isoform 1"
was found to be more prominent in the susceptible lines and "isoform
2" in the resistant lines. Bulk segregant analysis (BSA) with twenty-five
rice microsatellites (RM) resulted in identification of three polymorphic
markers between bulks RM11 and RM432 located on chromosome 7 and RM271 on
chromosome 10 of rice. These markers may be associated with leaffolder
resistance in rice and can be used in marker-assisted selection for leaffolder
resistance in rice.
 
Date 2015-01-16T11:38:21Z
2015-01-16T11:38:21Z
2005-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30384
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.42(4) [August 2005]