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CDC27 protein is involved in radiation response in squamous cell cervix Carcinoma

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Title CDC27 protein is involved in radiation response in squamous cell cervix Carcinoma
 
Creator Rajkumar, T
Gopal, G
Selvaluxrni, G
Rajalekshmy, K R
 
Subject cdc27
Cervix cancer
Predictive markers
Radiation response
Differential display
Prognosis
 
Description 271-278
In the present study, an attempt was made to
identify genes involved in radiatiorr response in cervix carcinoma. Differential
display technique was used to study the expression profiles of tumour biopsy
samples obtained from patients,

responding and not responding to treatment. The
samples were obtained prior to radiotherapy and subsequent to treatment with
Tele-radiation at 10 Gray (Gy). One of the differentially expressed cDNAs, when
sequenced was identified to be

CDC27. Immuno-histochemical analysis of pre- and
post-treated tumour samples from fifteen patients showed the downregulation of
expression of CDC27 protein in seven patients. Down-regulation was associated
with poorer response to

radiotherapy. Cervical cancer cell lines SiHa and
C33A were irradiated and their nuclei were stained for expression of CDC27 and
analyzed using fluorescent-activated cell sorting (FACS). Down-regulation of CDC27
protein in the irradiated SiHa cell line was associated with greater survival
fraction, compared to the irradiated C33A cell line, which had only slight fall
in the level of CDC27 protein. This is the first study to suggest a role for
CDC27 in radiation response. However, a

larger cohort is needed to further confirm the
value of CDC27 protein as a predictive marker, for radiation response in cervix
cancer.
 
Date 2015-01-16T11:40:14Z
2015-01-16T11:40:14Z
2005-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30386
 
Language en_US
 
Relation GOIN33/574
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.42(5) [October 2005]