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<span style="mso-bidi-language:HI">Rhizobial lipopolysaccharide as the receptor in lectin<i>-Rhizobium </i>interaction </span>

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Title Rhizobial lipopolysaccharide as the receptor in lectin-Rhizobium interaction
 
Creator Bhattacharya, Indranil
Biswas, Sagarika
Das, Rakha H
Das, Hasi R
 
Subject Lipopolysaccharide
Peanut root lectin
Exopolysaccharide
Receptor
Lectin-Rhizobium interaction
 
Description 89-95
Rhizobial specificity was examined on the basis of
interaction between legume lectins (peanut, pea and soybean) and different
rhizobial species (various bradyrhizobia specific for peanut, P 14-93 and SB16).
Legume lectins showed higher affinity towards host-specific Rhizobium and
lipopolysaccharides (LPS) isolated from those particular rhizobia. Two LPS mutants
of peanut-specific Bradyrhizobium sp. (Arachis) strain GN17
were isolated by Tn5 mutagenesis. These mutants (GN17MI and GNI7M2) were
characterized by their higher hydrophobicity with respect to the parent cells.
The hexose content in exopolysaccharides. (EPS) and LPS of the mutants was
found reduced significantly, whereas 2-keto-3-deoxyoctulosonic acid (Kdo) and
uronic acid in LPS were less by 20-times and thrice, respectively in the
mutants. Glucose was the major sugar in LPS from all the strains. However,
glucosamine appeared only in the mutants. Spectrofluorimetric analysis showed
that LPS from GN17Ml mutant interacted most significantly with peanut root
agglutinin or lectin (PRA II). The results indicate that LPS on the surface of
rhizobial cells is the possible receptor for lectin.
 
Date 2015-01-16T11:50:04Z
2015-01-16T11:50:04Z
2004-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30393
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.41(2&3) [April-June 2004]