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Structural alterations of phospholipid film domain morphology induced by cholesterol

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Title Structural alterations of phospholipid film domain morphology induced by cholesterol
 
Creator Panda, A K
Hume, A
Nag, K
Harbottle, R R
Petersen, N O
 
Description 114-121
Structures of the monolayer films of
dipalmitoylphosphatidylcholine (DPPC) mixed with different amounts of cholesterol
were studied at air-water interface using surface pressure-area measurements,
epifluorescence microscopy and atomic force microscopy (AFM). Pure DPPC,
cholesterol or DPPC-cholesterol mixtures were dissolved in organic solvents with
a small amount of fluorescently labeled phospholipid probe (NBD-PC) and spread
onto the air-water interface. Surface

pressure-area isotherms and epifluorescence
microscopy of such films at the air-water interface suggested that DPPC undergoes
a gas to fluid to condensed phase transition, while cholesterol undergoes a gas
to solid-like transition. A shift of the surface pressure-area curve to lower
area per molecule was observed when cholesterol was mixed with DPPC. Epifluorescence
microscopy showed the formation of spiral shaped domains for mixed monolayers.
Increase in cholesterol content abolished domain characteristics possibly due
to fluidizing property of cholesterol. AFM measurements of monolayers,
transferred onto freshly cleaved mica by Langmuir-Blodgett technique, revealed
the alterations caused by cholesterol on the gel and fluid domains of such
films. AFM measurements re-established similar trend in domain

characteristics as evidenced in epifluorescence
microscopy.
 
Date 2015-01-16T11:56:59Z
2015-01-16T11:56:59Z
2003-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30398
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.40(2) [April 2003]