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Lys-413 of S-phase mRNA cycling sequence binding protein from <i style="mso-bidi-font-style:normal">Leishmania donovani</i> (LdCSBP) is modified through monoubiquitination that is responsible for inhibition of its riboendonuclease activity

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Title Lys-413 of S-phase mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) is modified through monoubiquitination that is responsible for inhibition of its riboendonuclease activity
 
Creator Guha, Kasturi
Das, Sneha
Saha, Partha
 
Subject Ubiquitination
Monoubiquitination
Site of monoubiquitination
Leishmania donovani
RNA binding protein
RNA endonuclease
 
Description 559-566
In addition to
well-known process of proteasome-mediated degradation of polyubiquitinated
proteins, monoubiquitination of proteins is also an important
post-translational modification that regulates various non-degradative cellular
processes like protein trafficking, cellular signalling, DNA replication and
DNA repair. We have previously characterized a multi-domain cycling sequence
binding protein LdCSBP from Leishmania
donovani, which binds specifically to a conserved CAUAGAAG octamer
containing RNAs via its uniquely arranged CCCH type Zn-fingers and degrades
them using its Smr endonuclease domain, indicative of its potential role in the
turnover of the S-phase mRNAs. Remarkably, its riboendonuclease activity is
inhibited due to the incorporation of a monoubiquitin residue in the ZnF
domain, though the target Lys residue remains unknown. Here, we report through
systematic mutation of Lys residue to Ala that Lys-413 in LdCSBP is the site of
monoubiquitination. However, the amino acid motif around the target Lys in
LdCSBP is not consensus with any previously known monoubiquitination site,
though partial homology is observed with a subset of recently identified
mammalian ubiquitination target sites. Interestingly, Lys-413 of LdCSBP is
conserved in the homologous annotated proteins from the related kinetoplastida
parasites, suggesting similar monoubiquitination-mediated regulation of RNA
endonuclease activity in the organisms.
 
Date 2015-02-11T04:45:14Z
2015-02-11T04:45:14Z
2014-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/30507
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.51(6) [December 2014]