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Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition

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Title Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition
 
Creator Singh, Archana
Bhushan, Bharat
Gaikwad, Kishor
Yadav, O P
Kumar, Suresh
Rai, R D
 
Subject Antioxidant enzymes
Gene expression
High-affinity potassium transporter
Wheat
Salt tolerance
Triticum aestivum L.
 
Description 75-85
Plants,
being sessile in nature, have developed mechanisms to cope with high salt
concentrations in the soil. In this study, the effects of NaCl (50-200 mM) on expression of high-affinity
potassium transporters (HKTs), antioxidant enzymes and their isozyme
profiles were investigated in two contrasting bread wheat (Triticum aestivum L.)
genotypes viz., HD2329
(salt-sensitive) and Kharchia65 (salt-tolerant). Kharchia65
can successfully grow in salt affected soils, while HD2329 cannot
tolerate salt stress. Differential expression studies of two HKT genes (TaHKT2;1.1 and TaHKT2;3.1)
revealed their
up-regulated expression (~1.5-fold) in the salt-sensitive HD2329 and
down-regulated (~5-fold) inducible expression in the
salt-tolerant genotype (Kharchia65). Specific
activity of antioxidant enzymes, viz. superoxide dismutase (SOD), peroxidase
(POX), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase
(GR) was found to be higher in the salt-tolerant genotype. Isozyme profile of
two (POX and GR) antioxidant enzymes showed polymorphism between salt-tolerant
and
salt-sensitive genotypes. A new gene TaHKT2;3.1 was also identified and its expression profile and role in
salt stress tolerance in wheat was also studied. Partial sequences of the TaHKT2;1.1 and TaHKT2;3.1 genes from bread wheat were
submitted to the EMBL GenBank database. Our findings indicated that defence
responses to salt stress were induced differentially in contrasting bread wheat
genotypes which provide evidences for functional
correlation between salt stress tolerance and differential biochemical
and molecular expression patterns in bread wheat.
 
Date 2015-03-30T05:18:03Z
2015-03-30T05:18:03Z
2015-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/31265
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.52(1) [February 2015]