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Detection of DNA polymerase λ activity during seed germination and enhancement after salinity stress and dehydration in the plumules of indica rice (<i style="mso-bidi-font-style:normal">Oryza sativa</i> L.)

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Title Detection of DNA polymerase λ activity during seed germination and enhancement after salinity stress and dehydration in the plumules of indica rice (Oryza sativa L.)
 
Creator Sihi, Sayantani
Bakshi, Sankar
Sengupta, Dibyendu Narayan
 
Subject Oryza sativa indica rice
DNA pol λ
ddNTP sensitivity
Activity gel
Abiotic stress
 
Description 86-94
DNA polymerase λ (DNA pol λ) is the
only reported X-family DNA polymerases in plants and has been shown to play a
significant role in dry quiescent seeds, growth, development and nuclear DNA
repair. cDNA for DNA pol λ has been
reported in Arabidopsis and japonica rice cultivar and has been
characterized from E. coli expressed
protein, but very little is known about its activity at protein level in
plants. The enzymatic activity of DNA pol λ was studied in dry, imbibed and
during different germination stages of indica rice IR-8 (salt sensitive) by
in-gel activity assay to determine its physiological role in important stages
of growth and development. The upstream sequence was also analyzed using
plantCARE database and was found to contain several cis-acting elements, including light responsive elements,
dehydration responsive elements, Myb binding sites, etc. Hence, 4-day-old
germinating seedlings of IR29, a salt-sensitive, but high yielding indica rice
cultivar and Nonabokra, a salt-tolerant, but low yielding cultivar were treated
with water (control) or 250 mM NaCl or 20% polyethyleneglycol-6000 for 4 and 8
h. The protein was analyzed by in vitro
DNA pol λ activity assay, in-gel activity assay
and Western blot analysis. DNA pol λ was not
detected in dry seeds, but enhanced after imbibition and detectable from low
level to high level during subsequent germination steps. Both salinity and
dehydration stress led to the enhancement of the activity and protein level of
DNA pol λ, as compared to control tissues. This
is the first evidence of the salinity or dehydration stress induced enhancement
of DNA pol λ activity in the plumules of rice (Oryza sativa L.) cultivars.
 
Date 2015-03-30T05:20:41Z
2015-03-30T05:20:41Z
2015-03-30T05:20:41Z
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/31266
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Source IJBB Vol.52(1) [February 2015]