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<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">In silico</span></i><span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB"> structural and functional analysis of protein encoded by wheat early salt-stress response gene (WESR3)</span>

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Title In silico structural and functional analysis of protein encoded by wheat early salt-stress response gene (WESR3)
 
Creator Mishra, A K
Tandon, Gitanjali
Sharma, Rajendra
Chandrasekharan, H
Pandey, P S
 
Subject WESR3
Triticum aestivum L.
TaMlo
Abiotic stress
Wheat early salt-stress response gene
 
Description 95-100
Salt stress is
one of the major abiotic stresses limiting grain yield in wheat (Triticum
aestivum L.). Wheat
early salt-stress response gene (WESR3)
is one of the major salt stress genes, which is affected in the first phase of
salt stress. In this study, sequence and structural analysis of protein coded
by WESR3 gene was carried out using
various bioinformatics tools. Sequence analysis of WESR3 protein revealed the presence
of highly conserved regions of Mlo
gene family. Three-dimensional modeling was carried out to elucidate its
structure and its active site. The sequence analysis revealed that WESR3
protein might be involved in fungal pathogen attack pathway. Thus, in addition
to its involvement in abiotic stresses, it also seemed to play an important
part in biotic stress pathways. Out of the three modeled protein structures
obtained from I-TASSER, HHPred and QUARK, the I-TASSER protein model was the
best model based on high confidence score and lesser number of bad contacts.
The Ramchandran plot analysis also showed that all amino acid residues of
I-TASSER model lie in the allowed region and thus indicating towards the
overall good quality of the predicted model. Seventeen active sites were
predicted in the protein bearing resemblance to the Mlo family conserved
regions. In conclusion, a detailed analysis of WESR3 protein suggested an
important role of WESR3 in biotic and abiotic stress. These results aid
to the experimental data and help to build up a complete view of WESR3 proteins
and their role in plant stress response.


 
Date 2015-03-30T05:24:36Z
2015-03-30T05:24:36Z
2015-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/31267
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.52(1) [February 2015]