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Microarray analysis of differentially expressed genes regulating lipid metabolism during melanoma progression

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Title Microarray analysis of differentially expressed genes regulating lipid metabolism during melanoma progression
 
Creator Sumantran, Venil N
Mishra, Pratik
Sudhakar, N
 
Subject Microarray
Melanomas
Lipid metabolism
NCI-60 Cell Miner
KEGG pathway analysis
 
Description 125-131
A new hallmark of cancer involves
acquisition of a lipogenic
phenotype which promotes tumorigenesis. Little is known
about lipid metabolism in melanomas. Therefore, we used BRB (Biometrics
Research Branch) class comparison tool with multivariate analysis to identify differentially expressed genes in human cutaneous melanomas, compared with benign nevi and
normal skin derived from the microarray dataset (GDS1375). The methods were validated
by identifying known melanoma biomarkers (CITED1,
FGFR2, PTPRF, LICAM, SPP1 and PHACTR1)
in our results. Eighteen genes regulating metabolism of fatty acids, lipid second messengers and gangliosides were 2-9 fold upregulated in melanomas of GDS-1375.
Out of the 18 genes, 13 were confirmed by KEGG pathway analysis and 10 were
also significantly upregulated in human melanoma cell lines of NCI-60 Cell
Miner database. Results showed that melanomas upregulated PPARGC1A transcription
factor and its target genes regulating synthesis of fatty acids (SCD) and complex lipids (FABP3 and ACSL3). Melanoma also upregulated genes which prevented lipotoxicity (CPT2 and ACOT7) and regulated lipid second
messengers, such as phosphatidic acid (AGPAT-4,
PLD3) and inositol
triphosphate (ITPKB, ITPR3). Genes
for synthesis of pro-tumorigenic GM3 and GD3 gangliosides (UGCG, HEXA, ST3GAL5 and ST8SIA1) were also upregulated in melanoma. Overall,
the microarray analysis of GDS-1375 dataset indicated that melanomas can become lipogenic by
upregulating genes, leading to increase in fatty acid
metabolism, metabolism
of specific lipid second messengers, and ganglioside synthesis.
 
Date 2015-05-14T11:12:24Z
2015-05-14T11:12:24Z
2015-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/31522
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.52(2) [April 2015]