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<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Comparison of activity of OsDmc1A recombinase of rice (<i style="mso-bidi-font-style: normal">Oryza sativa)</i> in presence of Ca<sup>2+</sup> and Mg<sup>2+</sup> ions</span>

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Title Comparison of activity of OsDmc1A recombinase of rice (Oryza sativa) in presence of Ca2+ and Mg2+ ions
 
Creator Chittela, Rajani Kant
Melzer, Michael
Sainis, Jayashree Krishna
 
Subject OsDmc1 recombinase
Homologous recombination
FRET assay
Electron microscopy
Meiosis
Oryza sativa
Rice
 
Description 161-168
Recombinases
are known to play an important role in the homology search and strand exchange
during meiosis as well as homologous recombination (HR)-mediated DNA repair
specifically require Mg2+ ion for their activity. The Ca2+
has been shown to stimulate the strand exchange activity of hDmc1 and ScDmc1 by
forming the extended filaments on DNA.
Oryza sativa disrupted meiotic cDNA1A
(OsDmc1A), a homologue of yeast and human Dmc1 from rice shows the hallmark
functions of recombinase. Here, we report the effects of Ca2+ and Mg2+
on OsDmc1A activity from rice (Oryza
sativa). OsDmc1A showed a concentration-dependent binding with both
single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) substrates in
presence of Mg2+ or Ca2+. The ssDNA and dsDNA binding
activities, as well as renaturation activity of OsDmc1A were similar in the
presence of Ca2+ or Mg2+. Increasing the Ca2+
or Mg2+ increased the DNA binding, renaturation and strand exchange
of OsDmc1A. But, OsDmc1A showed only a slight stimulation of strand exchange
activity in presence of Ca2+, when compared the activity in presence
of Mg2+. Electron microscopy showed that OsDmc1A formed ring-like
structures in presence of Mg2+ or Ca2+. However, OsDmc1A
formed filament like structures with both ss and dsDNA in presence of Mg2+
or Ca2+. Taken together, Ca2+ did not affect OsDmc1A
recombinase activity significantly.
 
Date 2015-05-14T11:35:44Z
2015-05-14T11:35:44Z
2015-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/31527
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.52(2) [April 2015]