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<span style="mso-bidi-language:HI">Physico-chemical and antigenic characterization of unconventional heavy chain antibodies of Indian desert camel (<i>Camelus dromedarius </i>L.) </span>

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Title Physico-chemical and antigenic characterization of unconventional heavy chain antibodies of Indian desert camel (Camelus dromedarius L.)
 
Creator Sehrawat, Sharvan
Singh, Ajit
 
Subject Indian camel
Heavy chain antibodies
Purification
Electrophoresis
Antigenic relationship
Immunoblots
Camelus dromedarius L.
 
Description 299-304
Heavy chain antibodies (HCAbs) of IgG2 and IgG3
subtypes were purified from the sera of Indian desert camel (Camelus
dromedarius L.) by ammonium sulphate precipitation, followed by
ion-exchange chromatography on DEAE-cellulose and affinity chromatography on
protein A-sepharose and protein G-sepharose, and characterized by SOS-polyacrylamide
gel electrophoresis, agar gel immunodiffusion (AGIO),
counter-immunoelectrophoresis (CIEP), immunoelectrophoresis (IEP), ELISA and
immunoblotting. IgG2 and IgG3 were found to have molecular mass 46.77 kDa and
43.65 kDa, respectively by SOS-PAGE under reducing conditions. They migrated in
β-region in IEP and could be detected in CIEP, because of being more negatively
charged and smaller size. Anti-camel IgG3 cross-reacted in AGIO,

ELISA and immunoblotting with IgGs of pig and
ruminants (cattle, buffalo, sheep and goat), but not with immunoglobulins from
horse, dog, guinea pigs, mice, fish, poultry and human. The present findings
suggest close antigenic relationship of camels with pigs and ruminants.
 
Date 2016-02-24T09:01:27Z
2016-02-24T09:01:27Z
2004-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/33809
 
Language en_US
 
Relation C 07 K 16/06
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.41(6) [December 2004]