Record Details

Expression analysis of γ-tocopherol methyl transferase genes and α-tocopherol content in developing seeds of soybean [<i>Glycine max</i> (L.) Merr.]

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Expression analysis of γ-tocopherol methyl transferase genes and α-tocopherol content in developing seeds of soybean [Glycine max (L.) Merr.]
 
Creator Vinutha, T
Maheswari, Chirag
Bansal, Navita
Prashat, G Rama
Krishnan, Veda
Kumari, Sweta
Dahuja, Anil
Sachdev, Archana
Rai, RD
 
Subject Glycine max
Soybean seed
γ-TMT gene
α-Tocopherol
 
Description 267-273
Soybean, Glycine max (L.) Merr., besides being rich source of protein and oil, is a good source health promoting substances viz., isoflavones, anthocyanins and tocopherols, the latter are composed of four isoforms α-, β-, γ- and
δ-tocopherols. Due to the potent antioxidant property and nutraceutical relevance of α-tocopherol, increasing its levels endogenously by modulating γ-tocopherol methyl transferase (γ-TMT) gene expression has gained significant attention in recent years. Despite having γ-tocopherol as the predominant form, its conversion into highly active α-tocopherol is the limiting step in soybean seeds; the step which is catalyzed by γ-tocopherol methyl transferase enzyme. Based on the differences in amino acid sequence at NH2-domain, the γ-TMT is classified as γ-TMT1, γ-TMT2 and γ-TMT3. Expression of these γ-TMT genes was studied in developing soybean seeds of two contrasting genotypes with respect to α-tocopherol content, namely Bragg (‘high’) and DS-2706 (‘low’). Among γ-TMT, significantly higher (P < 0.05) expression (2-4.5 fold) of γ-TMT3 was observed in Bragg genotype than in DS-2706 at 50 days after flowering (DAF). A positive correlation was observed between γ-TMT3 gene expression and α-tocopherol accumulation (14 µg/g seed) in Bragg at 50 DAF than in DS-2706. The study may prove useful in greater understanding of temporal differences in the α-tocopherol accumulation and γ-TMT3 gene expression in seeds and the distinct roles of γ-TMT genes in soybean.
 
Date 2017-04-11T05:07:40Z
2017-04-11T05:07:40Z
2015-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/41193
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.52(3&4) [June-August 2015]