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Identification of novel starch synthase genes using <i>de novo</i> assembly and heat-induced expression and activity in developing wheat (<i>Triticum aestivum </i> L.)

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Title Identification of novel starch synthase genes using de novo assembly and heat-induced expression and activity in developing wheat (Triticum aestivum L.)
 
Creator Verma, Pooja
Kumar, Ranjeet R
Goswami, Suneha
Sharma, Sushil K
Kumar, Mahesh
Singh, Jyoti P
Dubey, Kavita
Pathak, Himanshu
Rai, Raj D
 
Subject Catalase
ClustalW alignment
Glucosyltransferase
Heat stress
Peroxidase
qRT-PCR
Starch
Total antioxidant capacity
 
Description 254-266
Terminal heat stress (HS) reduces quantity and quality of grains. Wheat is highly sensitive to HS and there is a wide diversity among different genotypes for HS-tolerance. The enzymes associated with starch biosynthesis are severely affected by HS, especially starch synthases (SS). Different isoforms of SS have been reported from cereals viz. rice, maize, etc.; however, limited information is available in the wheat. In this study, we investigated the effect of HS on contrasting wheat (Triticum aestivum L.) cultivars — HD2985 (thermotolerant) and HD2329 (thermosusceptible) at grain-filling stage. Here we report the identification and cloning of three putative SS genes from wheat. The putative SS has been characterized in silico with other SS reported from different plant and non-plant sources in order to investigate potential functional and evolutionary relationships between SSs. The correlation between HS and SS activity and other biochemical parameters associated with thermotolerance in two cultivars was also studied. Three putative SS genes (transcript_7326, transcript_24546 and transcript_38472) were cloned from HD2985 cultivar under HS by data mining generated from whole transcriptome analysis. The sequences were in silico characterized and submitted in NCBI GenBank (accession nos. KM206143, KM206144 and KJ854903). Glucosyltransferase domain was observed in all the three sequences. Based on ClustalW alignment, SSs were classified into four distinct families. Expression analysis and SS activity assay showed significantly higher transcript level and SS activity during mealy-ripe stage than at milky-ripe stage in HS-treated HD2985, compared to HD2329 cultivar. Both the cultivars showed decrease in starch content under HS and the overall content was higher in HD2985, compared to HD2329. Similarly, guaiacol peroxidase, catalase, free amino acid and total antioxidant capacity were higher during mealy-ripe stage in HD2985. HS during milky-ripe had more severe impact on the overall physiochemical properties of wheat grain and thus needed to be targeted using different approaches in order to enhance the quality and yield of wheat under HS.
 
Date 2017-04-11T05:16:31Z
2017-04-11T05:16:31Z
2015-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/41194
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.52(3&4) [June-August 2015]