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<i style="">In vitro</i> propagation of <i style="">Dendrobium microbulbon</i> A. Rich―A rare ethnomedicinal herb

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Title In vitro propagation of Dendrobium microbulbon A. Rich―A rare ethnomedicinal herb
 
Creator Sharma, Urvashi
Rao, V Rama
Mohan, J S S
Reddy, A S
 
Subject conservation
in vitro flowering
secondary explants
shoot multiplication
pseudobulbs
Dendrobium microbulbon
 
Description 381-384
Dendrobium microbulbon A. Rich (Orchidaceae) is a small epiphytic rare orchid used in stomachache by the tribal people of Gujarat. The present study deals with the development of a suitable protocol for its clonal propagation using pseudobulbs as explants. Murashige and Skoog (MS) basal medium supplemented with various concentrations of auxin and cytokinin was used either alone or in combination for induction, multiplication, elongation and rooting of in vitro shoots. MS medium fortified with 3% sucrose, 7.5 mg L-1 indole-3-acetic acid (IAA) and 20 mg L-1 benzyl aminopurine (BAP) showed axillary bud initiation. MS medium with 2.0 mg L-1 BAP favoured production of maximum number of shoots (39 shoots), elongation of shoots as well as bulblets formation. In vitro raised shoots were rooted on Knop salts with MS vitamins and MS iron salts. During acclimatization, 60% of the plantlets survived after their removal from in vitro conditions.
 
Date 2009-07-23T12:19:38Z
2009-07-23T12:19:38Z
2007-07
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://hdl.handle.net/123456789/5536
 
Language en_US
 
Relation Int. Cl.8 A01H4/00, 5/04
 
Publisher CSIR
 
Source IJBT Vol.6(3) [July 2007]