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<em>Mimosa pudica</em> L.: A comparative study via <em>in vitro</em> analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol

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Title Statement <em>Mimosa pudica</em> L.: A comparative study via <em>in vitro</em> analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
 
Added Entry - Uncontrolled Name T, Tasnuva S; International Islamic University malaysia
F, Sahena
A, Qamar U
G, Kashif
J, Juliana
ISM, Zaidul ; International Islamic University Malaysia
Ministry of Higher Education, Malaysia (MOHE)
 
Uncontrolled Index Term Medicinal chemistry; extraction techniques
Antioxidant, Conventional extraction, Derivetisation, GC Q-TOF MS, Mimosa pudica L., SFE
 
Summary, etc. The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely <em>Mimosa pudica</em> L. to obtain a safe antidiabetic natural agent. <em>In vitro</em> analysis comprised of two different assays, antioxidant assay (determination of total phenolic content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl assay) and antidiabetic assay (inhibition of α-amylase and α-glucosidase). GC Q-TOF MS profiling for both extracts was done after derivetisation to confirm the presence of bioactive compounds. SFE was performed at 40 MPa pressure, 60 °C temperature and 5 mL/min CO2 flow rate using 30 % ethanol (co-solvent) for 2 h. EtOHconv prepared using 95 % ethanol through conventional method (maceration) showed a good <em>in vitro</em> antioxidant potential and digestive enzymes inhibitory effect compared to supercritical fluid extract. α-amylase and α-glucosidase inhibitory activities for EtOHconv at 1 mg/mL were 30.08 % (±5.23) and 38.29 % (±2.52), whereas for standard acarbose it was 28.24 % (±13.66) and 36.93 % (±2.70), respectively. Supercritical fluid extract showed less potent <em>in vitro</em> antioxidant and digestive enzymes inhibitory effects (15.67±4.03- α-amylase, 28.36±2.01- α-glucosidase). GC Q-TOF MS analysis was done to confirm the presence of bioactive compounds in both the extracts. Although EtOHconv showed better results, SFE was found to contain more bioactive compounds associated with various pharmacological effects especially antioxidative as per GC Q-TOF MS results. SFE being a clean and green technology could be employed in future with more focus on method development and optimization to reproduce better and safe bioactive products from the neglected weed <em>M. pudica. <br /></em>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2017-06-02 15:25:38
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/13582
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 8, ##issue.no## 1 (2017): March 2017
 
Language Note en
 
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