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<p><span>Effect of extraction methods on yield, phytochemical constituents, antibacterial and antifungal activity of </span><em>Capsicum frutescens</em><span> L.</span></p>

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Title Statement <p><span>Effect of extraction methods on yield, phytochemical constituents, antibacterial and antifungal activity of </span><em>Capsicum frutescens</em><span> L.</span></p>
 
Added Entry - Uncontrolled Name Gurnani, Neelam ; School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain (M.P.), 456001, India
Gupta, Madhu ; Government Arts and Science College, Ratlam (M.P.), 457001, India
Shrivastava, Rakesh ; Department of Microbiology, RD Gardi Medical College, Ujjain (M.P.), 456001, India
Mehta, Darshana ; School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain (M.P.), 456001, India
Mehta, Bhupendra Kumar; School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain (M.P.), 456001, India
 
Uncontrolled Index Term Antimicrobial activity of spices; Comparison of conventional and modern extraction on antimicrobial activity of spice plant
Antibacterial, Antifungal, Capsicum frutescens L., Microwave, Minimum inhibitory concentration.
 
Summary, etc. <p><em><span lang="&quot;EN-GB&quot;">Capsicum frutescens</span></em><span lang="&quot;EN-GB&quot;"> L. (Family Solanaceae) commonly used to make one of the most common spice, red peppers is also associated with multiple health benefits. In the present study, extract yield and phytochemical constituents of <em>n</em>-hexane, chloroform, ethyl acetate, acetone and methanol extracts of dried seeds of <em>C. frutescens</em> L. prepared by microwave assisted solvent extraction technique, also known as “Green extraction” were compared with the solvent extracts prepared by two common conventional extraction methods. <em>In</em> <em>vitro</em> antibacterial and antifungal activity of all extracts were determined by using agar well diffusion method against three gram positive bacteria (<em>Bacillus cereus</em>, <em>Staphylococcus aureus</em> and <em>S. aureus MRSA</em><em>)</em>, five gram negative bacteria (<em>Escherechia coli</em>, <em>Salmonella typhi</em>, <em>Pseudomonas aeruginosa</em>,<em> Klebsiella pneumoniae</em> and <em>Proteus vulgaris</em><em><span>)</span></em> and two fungi (<em>Candida albicans</em> and <em>C. krusei</em><em>)</em>. Results were also compared with positive (Cotrimoxazole and Nystatin) and negative controls (Dimethyl sulphoxide). Solvent extracts prepared by microwave assisted method showed significant activity and <em><span>n</span></em>-hexane extract formed inhibition zone of 14.4 mm against <em>P.</em> <em><span>aeruginosa</span></em>, 20.0 mm against <em>C. albicans</em>, while ethyl acetate extract formed largest inhibition zone (15.0 mm) against <em>C.</em> <em><span>krusei</span></em>. Minimum inhibitory concentration evaluated by two fold serial broth tube dilution method ranged between 0.312 to 5 mg/mL.</span></p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2016-04-22 09:30:54
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/9138
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 7, ##issue.no## 1 (2016): March 2016
 
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