Record Details

<p><em>Ocimum sanctum </em><span>L. a potential angiotensin converting enzyme (ACE) inhibitor useful in hypertension</span></p>

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement <p><em>Ocimum sanctum </em><span>L. a potential angiotensin converting enzyme (ACE) inhibitor useful in hypertension</span></p>
 
Added Entry - Uncontrolled Name Chaudhury, Sushil Kumar
Mukherjee, Pulok K; Director School of Natural Product Studies Dept. of Pharmaceutical Technology Jadavpur University, Kolkata 700032, India Tele Fax: + 91 33 24146046 [Office] Telephone: +91 33 24298313 [Residence] Associate Editor Journal of ETHNOPHARMCOLOGY [Elsevier Science, Ireland]
Maity, Niladri
Nema, Neelesh K
Bhadra, Santanu
Saha, Bishnu Pada
Council of Scientific and Industrial Research (CSIR), Government of India [F.No. - 60(0084)/08/EMR-II/2009]
 
Uncontrolled Index Term Natural Product Research
ACE inhibition, Ocimum sanctum L., Eugenol, Antioxidant, HPLC.
 
Summary, etc. <p><span>The main objective of this study was to determine the </span><em>in vitro</em><span> Angiotensin Converting Enzyme (ACE) inhibition and antioxidant activity of standardized extract of the </span><em>Ocimum sanctum</em><span> L. leaves and its fractions. The ACE inhibition activity and antioxidant activity were investigated by UV method. In ACE inhibition method, the synthetic substrate hippuryl-L-histidyl-L-leucine was allowed to react with a test sample containing ACE, to produce hippuric acid. Dried hippuric acid was dissolved in distilled water, the absorbance of the solution was determined in the ultraviolet region and from the concentration of hippuric acid and the ACE inhibition activity was calculated. The antioxidant activity of the plant extract/fractions were examined on the basis of the scavenging effect on the stable DPPH free radical activity. Different concentrations of methanol extract of </span><em>O</em><span>. </span><em>sanctum</em><span> L. and eugenol were subjected to HPLC analysis using the mobile phase (methanol: water: acetic acid; 60 %: 40 %: 1 %). </span><em>O. sanctum </em><span>L. oil, eugenol and ethyl acetate fractions showed the maximum ACE inhibition activity in a concentration-dependent manner with IC</span><sub>50</sub><span> value of 32.11 ± 3.6, 42.16 ± 2.7 and 56.83 ± 2.8 µg/mL, respectively. Strong DPPH radical scavenging was also found in </span><em>O. sanctum</em><span> L. oil, methanol extract and ethyl acetate fractions, showing IC</span><sub>50</sub><span> value of 40.31± 3.5µg/mL, 105.62 ± 4.6 and 145.31 ± 5.8µg/mL, respectively. </span><em>O. sanctum </em><span>L. extract/fractions, eugenol and </span><em>O. sanctum</em><span> L</span><em>.</em><span> oil inhibited ACE in concentration-dependent manner.</span></p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2016-02-19 09:37:24
 
Electronic Location and Access "application/pdf"
http://op.niscair.res.in/index.php/IJNPR/article/view/1178
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 5, ##issue.no## 1 (2014): March 2014
 
Language Note en
 
Terms Governing Use and Reproduction Note Except where otherwise noted, the Articles on this site are licensed underCreative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India© 2012. The Council of Scientific &amp; Industrial Research, New Delhi.