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<p><span>Antibacterial and antioxidant activities of methanol extract and fractions of </span><em>Clerodendrum viscosum </em><span>Vent. leaves</span></p>

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Title Statement <p><span>Antibacterial and antioxidant activities of methanol extract and fractions of </span><em>Clerodendrum viscosum </em><span>Vent. leaves</span></p>
 
Added Entry - Uncontrolled Name Ghosh, Goutam ; School of Pharmaceutical Sciences, S O A University,
Sahoo, Suprava ; Centre of Biotechnology
Das, Debajyoti ; School of Pharmaceutical Sciences
Dubey, Debasmita
Padhy, Rabindra N
 
Uncontrolled Index Term Clerodendrum viscosum, Antimicrobial, Antioxidant activity, Total phenolic content
 
Summary, etc. <p><span>The present study was undertaken to evaluate antimicrobial and antioxidant activities of methanol extract as well as n-hexane, chloroform, ethyl acetate and aqueous fractions of </span><em>Clerodendrum viscosum</em><span> Vent</span><em>.</em><span> leaves. The antimicrobial activity, total phenolics and flavonoids were determined by agar-well diffusion, Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively The antioxidant potential was evaluated by using various </span><em>in vitro </em><span>methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), nitric oxide and hydrogen peroxide radical scavenging assays. The maximum inhibition zones for </span><em>S. aureus</em><span>, </span><em>B. subtilis, E. coli, K. pneumoniae </em><span>and</span><em> P. aeruginosa</em><span> were 27, 25, 28, 23 and 22 mm for ethyl acetate fraction, respectively, whereas n-hexane fraction of methanol extract showed the weakest antimicrobial activity with inhibitory effect against </span><em>B. subtilis</em><span> and </span><em>K. pneumoniae</em><span>. The total phenolic content (TPC) in ethyl acetate fraction (87.28 mg GAE/g) was highest, followed by methanol extract (76.75 mg GAE/g), aqueous fraction (61.36 mg GAE/g) and chloroform fraction (28.46 mg GAE/g), respectively. The total flavonoid content (TFC) was in order of ethyl acetate fraction (77.48 mg quercetin equivalent/ g) &gt; methanol extract (64.56 mg quercetin equivalent/ g) &gt; aqueous fraction (49.66 mg quercetin equivalent/g) &gt; chloroform fraction (32.85 mg quercetin equivalent/ g). The ethyl acetate fraction of methanol extract of </span><em>C. viscosum</em><span> leaves showed moderate free radical scavenging activity as evidenced by IC</span><sub>50</sub><span> values in DPPH (64.51μg/mL), ABTS (28.32μg/mL), nitric oxide (80.02μg/mL) and H</span><sub>2</sub><span>O</span><sub>2</sub><span> (77.45μg/mL) scavenging assays. The results of present study showed that </span><em>C. viscosum</em><span> leaves possess high phenolic, flavonoid contents, potential antimicrobial and antioxidant activity and could be a potential source of natural antioxidant that could have great importance as therapeutic agents in preventing or slowing the progress of ageing and age associated oxidative stress related degenerative diseases.</span></p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2016-02-19 10:05:16
 
Electronic Location and Access "application/pdf"
http://op.niscair.res.in/index.php/IJNPR/article/view/2001
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 5, ##issue.no## 2 (2014): June 2014
 
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