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Transcriptome and proteome study of brinjal (Solanum melongena L) during fruit development

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Title Transcriptome and proteome study of brinjal (Solanum melongena L) during fruit development
Ph.D.
 
Creator Israr Ahmad
 
Contributor Sharma, T. R.
 
Subject proteins, enzymes, fruits, vegetables, genes, biological development, transcription, planting, diseases, metabolism
 
Description brinjal or eggplant (Solanum melongena L.) is third most important
solanaceae crop species. In the present study, genome-wide transcriptome and
proteome analysis was carried out during different stages of fruit development in
brinjal cultivars Pusa Purple Long (PPL) and Killikulam (KKM-1) to understand the
molecular mechanisms involved in fruit development. In transcriptome analysis,
1395 and 1174 transcripts showed differential expression in brinjal cultivars PPL
and KKM-1 at various stages of fruit development, respectively. Maximum number
of transcripts (858, 752) showed differential expression at 50 dpa while least number
of transcripts (383, 311) showed differential expression at 5 dpa in PPL and KKM-1
cultivars. Microarray analysis showed that genes involved in cell cycle, cell growth,
cell wall biosynthesis like CycB1, CycB2, CycD3, tubulin, kinesin, XTR, FLA and
EXGT and brasinosteroid synthesis (HYD1, DWF1) were up-regulated during early
fruit development stages. The genes involved in photosynthesis, carbon and energy
metabolism and redox responsive genes were up-regulated throughout the fruit
development stages. Further, genes involved in ethylene biosynthesis were upregulated
at 20 dpa. MYB box and MADS box (AP3, AGL6) family TFs were
mainly down regulated while IAA/AUX (IAA7, IAA8 and IAA9) family TFs were upregulated
at all the stages except at ripening stage. The G protein and MPK signaling
were up-regulated while genes for receptor kinase and Ca signaling were downregulated.
Protein synthesis related gene was down-regulated while protein
degradation gene was up-regulated at the ripening stage. Proteomic analysis revealed
a total of 497 and 499 protein spots in brinjal cultivars PPL and KKM-1,
respectively of which 135 and 101 protein spots showed differential expression with
≥1.5 fold change. Proteins related to cell growth and cell wall metabolism like actin-
54, annexin p34, pectinesterase were up-regulated at all the stages except 5 dpa
while, proteins related to signaling and transport like 14-3-3 protein, V-type proton
ATPase, vacuolar H+ATPase were up-regulated at all the stages of fruit development
and ripening. Many antioxidant proteins like ascorbate peroxidase, superoxide
dismutase and proteins related to glycolytic pathway were up-regulated during fruit
development and ripening. Ethylene Responsive Factor (ERF) family of genes
119
encode transcriptional regulators with a variety of functions involved in the
developmental and physiological processes in plants. In this study expression
analysis of six SmERF genes during fruit development and ripening stages of brinjal
revealed that SmERF80 was highly up-regulated at 20 dpa and 50 dpa. Expression of
SmERF52 and SmERF83 was high at 5dpa but further down-regulated till ripening.
Expression of SmERF7, SmERF70 and SmERF80 was found to be high at ripening
stage (50 dpa). SmERF7, SmERF20, SmERF52, SmERF70 and SmERF83 genes play
important role at early stage of fruit development whereas SmERF7, SmERF70 and
SmERF80 are probably involved in the non-climacteric ripening of brinjal fruit. In
this study, several stage specific differentially expressed transcripts and proteins
involved in various metabolic pathways have been identified. Present findings are
important for the molecular understanding of fruit development and ripening in this
economically important solanaceous crop and will helps in the development of new
varieties using molecular breeding or genetic modification techniques.
 
Date 2016-03-09T17:36:33Z
2016-03-09T17:36:33Z
2013
 
Type Thesis
 
Identifier IARI, MOLECULAR BIOLOGY AND BIOTECHNOLOGY, NEW DELHI
http://krishikosh.egranth.ac.in/handle/1/65023
 
Language en_US
 
Format application/pdf