Record Details

Morphological and molecular variability among the isolates of sclerotium rolfsii sacc. from different host plants

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Morphological and molecular variability among the isolates of sclerotium rolfsii sacc. from different host plants
 
Creator K.C.Jyothi
 
Contributor Srikanth Kulkarni
 
Subject Plant Pathology
 
Description Sclerotium rolfsii Sacc. is a soil inhabitant, non-target, polyphagous and an ubiquitous
facultative parasite. Ten pathogenic isolates of S. rolfsii obtained from potato, groundnut,
tomato, chilli, sunflower, soybean, onion, lucerne and wheat plants were collected from Main
Agricultural Research Station, University of Agricultural Sciences, Dharwad.
Morphological character studied on potato dextrose agar revealed that, considerable
variation among the isolates. The colony diameter varied from 52.00 to 89.83 mm at 72 h of
incubation. The colour of sclerotia was light to dark brown size of sclerotia varied from 1.30
to 3.40 mm and they were spherical to round in shape. The test weight of 100 sclerotial
bodies was recorded between 53.00 to 383.30 mg and number of sclerotia per cm² were
ranged between 1.14 to 6.55.
The cross inoculation studies on different hosts revealed that, isolates of S. rolfsii on
potato, tomato and chilli were found to be highly virulent. Groundnut, sunflower, cotton and
onion were found to be moderately virulent whereas wheat, lucerne and isolates were less
virulent.
Isozyme studies revealed that, potato and tomato isolates exhibited extra bands in case
of peroxidase studies and onion isolate produced only one band in case of polyphenoloxidase
studies.
RAPD data distinguished the ten isolates into two major clusters A and B. Major
cluster A composed of isolates viz., WT, CN, CL, LN TO, PO, SF and GN. The results
revealed that, isolates of solanaceous and oil seed crops were closely related. Hence, the
results obtained from the cluster analysis revealed that, sub cluster group composed of
isolates which showed very less variability.
Carboxin fungicide affected the growth of all the isolates at the concentrations under
in vitro conditions.
 
Date 2016-10-24T19:00:42Z
2016-10-24T19:00:42Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81656
 
Format application/pdf
 
Publisher UAS, Dharwad