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BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF COWPEA MILD MOTTLE VIRUS ASSOCIATED WITH SOYBEAN MOSAIC DISEASE IN INDIA

KrishiKosh

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Title BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF COWPEA MILD MOTTLE VIRUS ASSOCIATED WITH SOYBEAN MOSAIC DISEASE IN INDIA
M Sc
 
Creator YADAV MANOJ KUMAR
 
Contributor Kajal Kumar Biswas
 
Subject weeds, heterocyclic compounds, sugarcane, control methods, crops, cultivation, planting, yields, harvesting, biological development
 
Description T-8640
Cowpea mild mottle virus (CPMMV), a member of genus Carlavirus (family Betaflexiviridae), is transmitted by whitefly, Bemisia tabaci in non-persistent manner. CPMMV contains flexuous virus particle of 650 x 13 nm in size and its genome consists of single stranded positive sense RNA, about 8.12 kb. Soybean cultivars grown at Indian Agricultural Research Institute (IARI), New Delhi experimental field were found to be severely affected by CPMMV with estimated disease incidence of 18.6-71.2%, producing various kinds of symptoms including mosaic, mottling, stunting, blistering and varied degrees of leaf deformation. Host range of virus was studied on Leguminosae and Solanaceae family including economic crops, soybean (26 cultivars), French bean (five cultivars), mungbean, urdbean, cowpea and experimental hosts, tobacco, fenugreek and asparagus bean. Interestingly, all the 26 soybean cultivars tested were found to be susceptible to CPMMV after sap inoculation and transmission efficiency of this virus was found to vary from 25 to 100% depending on the soybean cultivars. Symptoms appeared in the infected plant 10-14 days after sap inoculation. Seed transmission was observed in different cultivars of soybean and efficiency ranged from 0.62 to 14.2%. Symptomatic leaves were found to contain long flexuous particle of 620-650 x 12-15 nm in size in leaf-dip preparation under electron microscopy. Carlavirus group specific universal primers amplifying 940 nt fragment from viral genome in RT-PCR, detected the associated virus causing mosaic/mottle disease of soybean in Delhi conditions to be a member of genus Carlavirus. Further, virus specific another set of primers confirmed the associated virus is CPMMV and designated as CPMMV soybean Delhi isolate (CPMMV-D1). Present study ruled out the Soybean mosaic virus (SMV) etiology associated with mosaic/mottle disease and revealed that this disease was caused by CPMMV of genus Carlavirus in soybean under Delhi condition.
 
Date 2016-09-19T17:26:13Z
2016-09-19T17:26:13Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/77529
 
Format application/pdf
 
Publisher IARI, Division of Plant Pathology