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Agrobacterium mediated transformation of indica rice variety IR-72 using Amaranthus seed albumin gene (AmA1) driven by a seed specific promoter

KrishiKosh

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Title Agrobacterium mediated transformation of indica rice variety IR-72 using Amaranthus seed albumin gene (AmA1) driven by a seed specific promoter
 
Creator Chowdhary, Ritika
 
Contributor Jain, R.K.
 
Subject Rice, Transformation, AmA1 gene, seed specific promoter
 
Description Experiments were conducted to transfer Amaranthus seed
albumin gene (AmA1) driven by GBSS promoter in indica rice variety IR-72
and Pusa Basmati by Agrobacterium-mediated transformation. During the
present investigation, 14-18 days old calli induced from mature seed scutella
and immature embryos on modified MS medium were used. The calli were
II
co-cultivated for 10 minutes in liquid co-cultivation medium with
Agrobacterium strain EHA105 (pSB8G) and then transferred onto the
solidified co-cultivation medium for 2-3 days. Calli were screened on selection
medium containing cefotaxime (250 mg/l) and G-418 (50 mg/l) for 2-3 cycles
of selection of 15 days each. A total of 160/1790 (mature seed) and 37/213
(immature embryo) calli of IR-72 and 124/831 of Pusa Basmati 1 showed
sustained proliferation on the selection medium. A total of 22 IR72 and three
Pusa Basmati 1 plants were regenerated from the selected calli by using the
MS based regeneration media supplemented with antibiotics. Fifteen of these
plants (IR72, 12 plants; Pusa Basmati 1, three plants) were successfully
transferred to the transgenic greenhouse. Five of the seven-rice plants
showed the amplification of 1.1 kb fragment indicating the presence of AmA1
gene. One of these PCR positive plants has already flowered and set seeds.
 
Date 2016-11-22T12:19:42Z
2016-11-22T12:19:42Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/86914
 
Language en
 
Format application/pdf
 
Publisher CCSHAU